Zc3h13/Flacc is required for adenosine methylation by bridging the mRNA binding factor Rbm15/Spenito to the m6A machinery component Wtap/Fl(2)d

Philip Knuckles, Tina Lence, Irmgard Haussmann, Dominik Jacob, Nastasja Kreim, Sarah H. Carl, Irene Masiello, Tina Hares, Rodrigo Villaseñor, Daniel Hess, Miguel A. Andrade-Navarro, Marco Biggiogera, Mark Helm, Matthias Soller, Marc Bühler, Jean-Yves Roignant

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N6-methyladenosine (m6A) is the most abundant mRNA modification in eukaryotes, playing crucial roles in multiple biological processes. m6A is catalyzed by the activity of Mettl3, which depends on additional proteins whose precise functions remain poorly understood. Here we identified Flacc/Zc3h13 as a novel interactor of m6A methyltransferase complex components in Drosophila and mouse. Like other components of this complex, Flacc controls m6A levels and is involved in sex
determination in Drosophila. We demonstrate that Flacc promotes m6A deposition by bridging Fl(2)d to the mRNA binding factor Nito. Altogether, our work advances our molecular understanding of conservation and regulation of the m6A machinery.
Original languageEnglish
Pages (from-to)415-429
Number of pages15
JournalGenes & Development
Early online date13 Mar 2018
Publication statusPublished - 2018


Bibliographical note

This article, published in Genes & Development, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.


  • Zc3h13
  • Flacc
  • m6A
  • methyltransferase complex
  • RNA modifications
  • sex determination

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