Simulated body fluid (SBF) has been used previously by others as an aid in predicting the bioactivity and osseointegration potential of materials. This paper details a study carried out using a CO2 laser to induce a number of surface patterns which inherently modified both the surface chemistry and surface topography of nylon 6,6 and gave rise to a difference in apatite response. These induced patterns caused a reduction in hydrophilicity with contact angles of up to +10° being observed. Furthermore, following immersion in SBF for 14 days, each sample was weighed revealing an increase in weight of up to 0.029 g indicating that an apatite layer had begun to form. In addition, energy dispersive X-ray (EDX) analysis identified the presence of calcium and phosphorous, two elements which support osteoblast cell response. When comparing with an as-received sample, it was found that the laser induced patterned samples gave rise to more layer crystals forming suggesting a more optimized surface for osteoblast cell growth and proliferation.