Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species

O. Sparagano, E. Drouet, R. Brebant, E. Manet, G. A. Denoyel, P. Pernin

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Monoclonal antibodies (MAbs) reactive to the pathogenic amoeba Naegleria fowleri were analyzed by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, Western blotting (immunoblotting), and radioimmunoprecipitation assay (RIPA). Two MAbs (3A4 and 5D12) showed reactivity by ELISA with all N. fowleri strains tested and no reactivity with the five other Naegleria species, N. lovaniensis, N. gruberi, N. australiensis, N. jadini, and N. andersoni. These MAbs reacted with the three morphological forms of N. fowleri (trophozoites, cysts, and flagellates). The reactivity on Western blots was suppressed by treatment with metaperiodate, suggesting a carbohydrate epitope. Differences in reactivity patterns between trophozoites and cysts observed with radioimmunoprecipitation assay might reflect differences in biological properties. The formalin stability of the epitope may be useful in detecting N. fowleri in fixed biopsies and in investigating the pathological process.

Original languageEnglish
Pages (from-to)2758-2763
Number of pages6
JournalJournal of Clinical Microbiology
Volume31
Issue number10
Publication statusPublished - 1 Jan 1993
Externally publishedYes

Fingerprint

Naegleria fowleri
Naegleria
Trophozoites
Cysts
Radioimmunoprecipitation Assay
Monoclonal Antibodies
Epitopes
Western Blotting
Enzyme-Linked Immunosorbent Assay
Amoeba
Pathologic Processes
Indirect Fluorescent Antibody Technique
Formaldehyde
Carbohydrates
Biopsy

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species. / Sparagano, O.; Drouet, E.; Brebant, R.; Manet, E.; Denoyel, G. A.; Pernin, P.

In: Journal of Clinical Microbiology, Vol. 31, No. 10, 01.01.1993, p. 2758-2763.

Research output: Contribution to journalArticle

Sparagano, O. ; Drouet, E. ; Brebant, R. ; Manet, E. ; Denoyel, G. A. ; Pernin, P. / Use of monoclonal antibodies to distinguish pathogenic Naegleria fowleri (cysts, trophozoites, or flagellate forms) from other Naegleria species. In: Journal of Clinical Microbiology. 1993 ; Vol. 31, No. 10. pp. 2758-2763.
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