Tunable Terpolymer Series for the Systematic Investigation of Membrane Proteins

Gestél C Kuyler; Elaine Barnard; Pooja Sridhar; Rebecca J Murray; Naomi L Pollock; Mark Wheatley; Timothy R Dafforn; Bert Klumperman

doi:10.1021/acs.biomac.4c01219

Tunable Terpolymer Series for the Systematic Investigation of Membrane Proteins

Gestél C Kuyler
, Elaine Barnard
, Pooja Sridhar
, Rebecca J Murray
, Naomi L Pollock
, Mark Wheatley
, Timothy R Dafforn
, Bert Klumperman

Stellenbosch University
University of Birmingham

Research output: Contribution to journal › Article › peer-review

9 Citations (Scopus)

90 Downloads (Pure)

Abstract

Membrane proteins (MPs) are critical to cellular processes and serve as essential therapeutic targets. However, their isolation and characterization are often impeded by traditional detergent-based methods, which can compromise their native states, and retention of their native lipid environment. Amphiphilic polymers have emerged as effective alternatives, enabling the formation of nanoscale discs that preserve MPs' structural and functional integrity. We introduce a novel series of poly(styrene- -maleic acid- -( -benzyl)maleimide) (BzAM) terpolymers with tunable amphiphilicity, synthesized through controlled polymerization. Designed to mimic and improve upon industry-standard poly(styrene- -maleic acid), these well-defined terpolymers offer enhanced control over molecular weight and distribution, allowing for systematic evaluation of polymer properties and their effect on membrane solubilization. The BzAM series effectively solubilized membranes and demonstrated a direct correlation between polymer hydrophob

Original language	English
Pages (from-to)	415-427
Number of pages	13
Journal	Biomacromolecules
Volume	26
Issue number	1
Early online date	26 Dec 2024
DOIs	https://doi.org/10.1021/acs.biomac.4c01219
Publication status	E-pub ahead of print - 26 Dec 2024

Bibliographical note

Copyright © 2024 The Authors. Published by American Chemical Society. This publication is licensed under
CC-BY 4.0 .

Funding

The authors acknowledge funding from the Wellcome Trust via a Technology Development grant entitled \u201CUnshackling Membrane Protein Research: New Amphiphilic Copolymers for Extraction of Stable, Active Membrane Proteins\u201D (Grant No. 223728/Z/21/Z). We are grateful for the contributions made by Dr Stephanie Nestorow and Bethan Kelly during the initial evaluations of the BzAM series.

Funders	Funder number
Wellcome Trust	223728/Z/21/Z
Wellcome Trust

Keywords

Copolymers
Hydrophobicity
Membranes
Polymers
Solubilization

Access to Document

10.1021/acs.biomac.4c01219Licence: CC BY

Murray2024VoRFinal published version, 3.05 MBLicence: CC BY

Cite this

@article{c82e7dac98ef451487eb05235585134f,

title = "Tunable Terpolymer Series for the Systematic Investigation of Membrane Proteins",

abstract = "Membrane proteins (MPs) are critical to cellular processes and serve as essential therapeutic targets. However, their isolation and characterization are often impeded by traditional detergent-based methods, which can compromise their native states, and retention of their native lipid environment. Amphiphilic polymers have emerged as effective alternatives, enabling the formation of nanoscale discs that preserve MPs' structural and functional integrity. We introduce a novel series of poly(styrene- -maleic acid- -( -benzyl)maleimide) (BzAM) terpolymers with tunable amphiphilicity, synthesized through controlled polymerization. Designed to mimic and improve upon industry-standard poly(styrene- -maleic acid), these well-defined terpolymers offer enhanced control over molecular weight and distribution, allowing for systematic evaluation of polymer properties and their effect on membrane solubilization. The BzAM series effectively solubilized membranes and demonstrated a direct correlation between polymer hydrophob",

keywords = "Copolymers, Hydrophobicity, Membranes, Polymers, Solubilization",

author = "Kuyler, \{Gest{\'e}l C\} and Elaine Barnard and Pooja Sridhar and Murray, \{Rebecca J\} and Pollock, \{Naomi L\} and Mark Wheatley and Dafforn, \{Timothy R\} and Bert Klumperman",

note = "Copyright {\textcopyright} 2024 The Authors. Published by American Chemical Society. This publication is licensed under CC-BY 4.0 .",

year = "2024",

month = dec,

day = "26",

doi = "10.1021/acs.biomac.4c01219",

language = "English",

volume = "26",

pages = "415--427",

journal = "Biomacromolecules",

issn = "1525-7797",

publisher = "American Chemical Society",

number = "1",

}

TY - JOUR

T1 - Tunable Terpolymer Series for the Systematic Investigation of Membrane Proteins

AU - Kuyler, Gestél C

AU - Barnard, Elaine

AU - Sridhar, Pooja

AU - Murray, Rebecca J

AU - Pollock, Naomi L

AU - Wheatley, Mark

AU - Dafforn, Timothy R

AU - Klumperman, Bert

PY - 2024/12/26

Y1 - 2024/12/26

N2 - Membrane proteins (MPs) are critical to cellular processes and serve as essential therapeutic targets. However, their isolation and characterization are often impeded by traditional detergent-based methods, which can compromise their native states, and retention of their native lipid environment. Amphiphilic polymers have emerged as effective alternatives, enabling the formation of nanoscale discs that preserve MPs' structural and functional integrity. We introduce a novel series of poly(styrene- -maleic acid- -( -benzyl)maleimide) (BzAM) terpolymers with tunable amphiphilicity, synthesized through controlled polymerization. Designed to mimic and improve upon industry-standard poly(styrene- -maleic acid), these well-defined terpolymers offer enhanced control over molecular weight and distribution, allowing for systematic evaluation of polymer properties and their effect on membrane solubilization. The BzAM series effectively solubilized membranes and demonstrated a direct correlation between polymer hydrophob

AB - Membrane proteins (MPs) are critical to cellular processes and serve as essential therapeutic targets. However, their isolation and characterization are often impeded by traditional detergent-based methods, which can compromise their native states, and retention of their native lipid environment. Amphiphilic polymers have emerged as effective alternatives, enabling the formation of nanoscale discs that preserve MPs' structural and functional integrity. We introduce a novel series of poly(styrene- -maleic acid- -( -benzyl)maleimide) (BzAM) terpolymers with tunable amphiphilicity, synthesized through controlled polymerization. Designed to mimic and improve upon industry-standard poly(styrene- -maleic acid), these well-defined terpolymers offer enhanced control over molecular weight and distribution, allowing for systematic evaluation of polymer properties and their effect on membrane solubilization. The BzAM series effectively solubilized membranes and demonstrated a direct correlation between polymer hydrophob

KW - Copolymers

KW - Hydrophobicity

KW - Membranes

KW - Polymers

KW - Solubilization

UR - https://www.scopus.com/pages/publications/85213475799

U2 - 10.1021/acs.biomac.4c01219

DO - 10.1021/acs.biomac.4c01219

M3 - Article

C2 - 39725644

SN - 1525-7797

VL - 26

SP - 415

EP - 427

JO - Biomacromolecules

JF - Biomacromolecules

IS - 1

ER -

Tunable Terpolymer Series for the Systematic Investigation of Membrane Proteins

Abstract

Bibliographical note

Funding

Keywords

Access to Document

Other files and links

Fingerprint

Cite this