Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan

Immo Fiebrig, Stephen E. Harding, Arthur J. Rowe, Stefan C. Hyman, Stanley S. Davis

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

Transmission electron microscopy has been employed to visualize the molecular structure and organization of a highly purified preparation of pig gastric mucin (molar mass M~9 × 106g/mol, as determined by low speed sedimentation equilibrium), prepared for microscopy by two completely independent methods. Samples were prepared for imaging by air drying on mica (in the presence of 50% glycerol) as well as critical point drying in acetone/CO2. The data appear consistent with the accepted linear model for mucins, and are consistent with regions of variable degrees of glycosylation along the polypeptide backbone chain, with the overall conformation that of a loose or random coil. The behaviour of this mucin in a dilute solution mixture with the potential mucoadhesive polymer chitosan (M~1 60,000 g/mol) was then explored, and a clear interaction was demonstrated, consistent with dilute solution measurements using sedimentation velocity analysis in the analytical ultracentrifuge.

Original languageEnglish
Pages (from-to)239-244
Number of pages6
JournalCarbohydrate Polymers
Volume28
Issue number3
DOIs
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Gastric Mucins
mucins
Chitosan
Mucins
chitosan
Transmission Electron Microscopy
Sedimentation
transmission electron microscopy
Drying
stomach
Swine
Transmission electron microscopy
Glycosylation
swine
Molar mass
Polypeptides
Mica
Acetone
Molecular Structure
Glycerol

ASJC Scopus subject areas

  • Organic Chemistry
  • Materials Chemistry
  • Polymers and Plastics
  • Food Science
  • Biochemistry

Cite this

Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan. / Fiebrig, Immo; Harding, Stephen E.; Rowe, Arthur J.; Hyman, Stefan C.; Davis, Stanley S.

In: Carbohydrate Polymers, Vol. 28, No. 3, 1995, p. 239-244.

Research output: Contribution to journalArticle

Fiebrig, Immo ; Harding, Stephen E. ; Rowe, Arthur J. ; Hyman, Stefan C. ; Davis, Stanley S. / Transmission electron microscopy studies on pig gastric mucin and its interactions with chitosan. In: Carbohydrate Polymers. 1995 ; Vol. 28, No. 3. pp. 239-244.
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