Transgenic analysis of sugar beet xyloglucan endotransglucosylase/hydrolase Bv-XTH1 and Bv-XTH2 promoters reveals overlapping tissue-specific and wound-inducible expression profiles: Transgenic analysis of sugar beetXTHgenes

E Dimmer, L Roden, Daguang Cai, Crawford Kingsnorth, Effie Mutasa‐Göttgens

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The identification and analysis of tissue‐specific gene regulatory elements will improve our knowledge of the molecular mechanisms that control the growth and development of different plant tissues and offer potentially useful tools for the genetic engineering of plants. A polymerase chain reaction (PCR)‐based 5′‐genome walk from sequences of an isolated sugar beet xyloglucan endo‐transglucosylase hydrolase (XTH) gene led to the isolation of two independent upstream fragments. They were 1332 and 2163 base pairs upstream of the XTH ATG start site, respectively. In vivo transgenic assays in sugar beet hairy roots and Arabidopsis thaliana revealed that both fragments had promoter function and, in A. thaliana, directed expression in vascular tissues within the root, leaves and petals. Promoter activity was also observed in the leaf trichomes and within rapidly expanding stem internodes. Expression driven by both promoters was found to be wound inducible. Overall, the spatial and temporal expression pattern of these promoters suggested that the corresponding Bv‐XTH genes (designated Bv‐XTH1 and Bv‐XTH2) may be involved in secondary cell wall formation. This work provides new insights on molecular mechanisms that could be exploited for the genetic engineering of sugar beet crops.
Original languageEnglish
Pages (from-to)127-139
Number of pages13
JournalPlant Biotechnology Journal
Volume2
Issue number2
DOIs
Publication statusPublished - 3 Feb 2004
Externally publishedYes

Fingerprint

xyloglucan:xyloglucosyl transferase
plant damage
hydrolases
sugar beet
promoter regions
genetically modified organisms
sugars
genetic engineering
Arabidopsis thaliana
vascular tissues
regulator genes
internodes
trichomes
corolla
plant tissues
leaves
growth and development
genes
polymerase chain reaction
cell walls

Bibliographical note

An Open Access journal

Keywords

  • genetic engineering
  • sugar beet
  • tissue-specific promoter
  • XTH

Cite this

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title = "Transgenic analysis of sugar beet xyloglucan endotransglucosylase/hydrolase Bv-XTH1 and Bv-XTH2 promoters reveals overlapping tissue-specific and wound-inducible expression profiles: Transgenic analysis of sugar beetXTHgenes",
abstract = "The identification and analysis of tissue‐specific gene regulatory elements will improve our knowledge of the molecular mechanisms that control the growth and development of different plant tissues and offer potentially useful tools for the genetic engineering of plants. A polymerase chain reaction (PCR)‐based 5′‐genome walk from sequences of an isolated sugar beet xyloglucan endo‐transglucosylase hydrolase (XTH) gene led to the isolation of two independent upstream fragments. They were 1332 and 2163 base pairs upstream of the XTH ATG start site, respectively. In vivo transgenic assays in sugar beet hairy roots and Arabidopsis thaliana revealed that both fragments had promoter function and, in A. thaliana, directed expression in vascular tissues within the root, leaves and petals. Promoter activity was also observed in the leaf trichomes and within rapidly expanding stem internodes. Expression driven by both promoters was found to be wound inducible. Overall, the spatial and temporal expression pattern of these promoters suggested that the corresponding Bv‐XTH genes (designated Bv‐XTH1 and Bv‐XTH2) may be involved in secondary cell wall formation. This work provides new insights on molecular mechanisms that could be exploited for the genetic engineering of sugar beet crops.",
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author = "E Dimmer and L Roden and Daguang Cai and Crawford Kingsnorth and Effie Mutasa‐G{\"o}ttgens",
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T1 - Transgenic analysis of sugar beet xyloglucan endotransglucosylase/hydrolase Bv-XTH1 and Bv-XTH2 promoters reveals overlapping tissue-specific and wound-inducible expression profiles

T2 - Transgenic analysis of sugar beetXTHgenes

AU - Dimmer, E

AU - Roden, L

AU - Cai, Daguang

AU - Kingsnorth, Crawford

AU - Mutasa‐Göttgens, Effie

N1 - An Open Access journal

PY - 2004/2/3

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N2 - The identification and analysis of tissue‐specific gene regulatory elements will improve our knowledge of the molecular mechanisms that control the growth and development of different plant tissues and offer potentially useful tools for the genetic engineering of plants. A polymerase chain reaction (PCR)‐based 5′‐genome walk from sequences of an isolated sugar beet xyloglucan endo‐transglucosylase hydrolase (XTH) gene led to the isolation of two independent upstream fragments. They were 1332 and 2163 base pairs upstream of the XTH ATG start site, respectively. In vivo transgenic assays in sugar beet hairy roots and Arabidopsis thaliana revealed that both fragments had promoter function and, in A. thaliana, directed expression in vascular tissues within the root, leaves and petals. Promoter activity was also observed in the leaf trichomes and within rapidly expanding stem internodes. Expression driven by both promoters was found to be wound inducible. Overall, the spatial and temporal expression pattern of these promoters suggested that the corresponding Bv‐XTH genes (designated Bv‐XTH1 and Bv‐XTH2) may be involved in secondary cell wall formation. This work provides new insights on molecular mechanisms that could be exploited for the genetic engineering of sugar beet crops.

AB - The identification and analysis of tissue‐specific gene regulatory elements will improve our knowledge of the molecular mechanisms that control the growth and development of different plant tissues and offer potentially useful tools for the genetic engineering of plants. A polymerase chain reaction (PCR)‐based 5′‐genome walk from sequences of an isolated sugar beet xyloglucan endo‐transglucosylase hydrolase (XTH) gene led to the isolation of two independent upstream fragments. They were 1332 and 2163 base pairs upstream of the XTH ATG start site, respectively. In vivo transgenic assays in sugar beet hairy roots and Arabidopsis thaliana revealed that both fragments had promoter function and, in A. thaliana, directed expression in vascular tissues within the root, leaves and petals. Promoter activity was also observed in the leaf trichomes and within rapidly expanding stem internodes. Expression driven by both promoters was found to be wound inducible. Overall, the spatial and temporal expression pattern of these promoters suggested that the corresponding Bv‐XTH genes (designated Bv‐XTH1 and Bv‐XTH2) may be involved in secondary cell wall formation. This work provides new insights on molecular mechanisms that could be exploited for the genetic engineering of sugar beet crops.

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DO - 10.1046/j.1467-7652.2004.00056.x

M3 - Article

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SP - 127

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