Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy

Lauren E. Jamieson, Jennifer Greaves, Jayde A. McLellan, Kevin R. Munro, Nicholas C.O. Tomkinson, Luke H. Chamberlain, Karen Faulds, Duncan Graham

Research output: Contribution to journalArticle

1 Citation (Scopus)
13 Downloads (Pure)

Abstract

Intracellular uptake, distribution and metabolism of lipids are tightly regulated characteristics in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells were monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regard to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging.

Original languageEnglish
Pages (from-to)30-36
Number of pages7
JournalSpectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
Volume197
Early online date7 Mar 2018
DOIs
Publication statusPublished - 15 May 2018
Externally publishedYes

Fingerprint

Alkynes
alkynes
fatty acids
Fatty acids
Lipids
Raman spectroscopy
Fatty Acids
lipids
Metabolism
metabolism
Fluorophores
cells
Fluorescence
Cells
Imaging techniques
fluorescence

Keywords

  • Alkyne tag
  • Fatty acids
  • Intracellular imaging
  • Lipids
  • Raman
  • Spectroscopy

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

Cite this

Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy. / Jamieson, Lauren E.; Greaves, Jennifer; McLellan, Jayde A.; Munro, Kevin R.; Tomkinson, Nicholas C.O.; Chamberlain, Luke H.; Faulds, Karen; Graham, Duncan.

In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy, Vol. 197, 15.05.2018, p. 30-36.

Research output: Contribution to journalArticle

Jamieson, Lauren E. ; Greaves, Jennifer ; McLellan, Jayde A. ; Munro, Kevin R. ; Tomkinson, Nicholas C.O. ; Chamberlain, Luke H. ; Faulds, Karen ; Graham, Duncan. / Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy. In: Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy. 2018 ; Vol. 197. pp. 30-36.
@article{ad6c427f38e74a138f4cc05d22468264,
title = "Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy",
abstract = "Intracellular uptake, distribution and metabolism of lipids are tightly regulated characteristics in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells were monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regard to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging.",
keywords = "Alkyne tag, Fatty acids, Intracellular imaging, Lipids, Raman, Spectroscopy",
author = "Jamieson, {Lauren E.} and Jennifer Greaves and McLellan, {Jayde A.} and Munro, {Kevin R.} and Tomkinson, {Nicholas C.O.} and Chamberlain, {Luke H.} and Karen Faulds and Duncan Graham",
year = "2018",
month = "5",
day = "15",
doi = "10.1016/j.saa.2018.01.064",
language = "English",
volume = "197",
pages = "30--36",
journal = "Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy",
issn = "1386-1425",
publisher = "Elsevier",

}

TY - JOUR

T1 - Tracking intracellular uptake and localisation of alkyne tagged fatty acids using Raman spectroscopy

AU - Jamieson, Lauren E.

AU - Greaves, Jennifer

AU - McLellan, Jayde A.

AU - Munro, Kevin R.

AU - Tomkinson, Nicholas C.O.

AU - Chamberlain, Luke H.

AU - Faulds, Karen

AU - Graham, Duncan

PY - 2018/5/15

Y1 - 2018/5/15

N2 - Intracellular uptake, distribution and metabolism of lipids are tightly regulated characteristics in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells were monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regard to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging.

AB - Intracellular uptake, distribution and metabolism of lipids are tightly regulated characteristics in healthy cells. An analytical technique capable of understanding these characteristics with a high level of species specificity in a minimally invasive manner is highly desirable in order to understand better how these become disrupted during disease. In this study, the uptake and distribution of three different alkyne tagged fatty acids in single cells were monitored and compared, highlighting the ability of Raman spectroscopy combined with alkyne tags for better understanding of the fine details with regard to uptake, distribution and metabolism of very chemically specific lipid species. This indicates the promise of using Raman spectroscopy directly with alkyne tagged lipids for cellular studies as opposed to subsequently clicking of a fluorophore onto the alkyne for fluorescence imaging.

KW - Alkyne tag

KW - Fatty acids

KW - Intracellular imaging

KW - Lipids

KW - Raman

KW - Spectroscopy

UR - http://www.scopus.com/inward/record.url?scp=85042911150&partnerID=8YFLogxK

U2 - 10.1016/j.saa.2018.01.064

DO - 10.1016/j.saa.2018.01.064

M3 - Article

VL - 197

SP - 30

EP - 36

JO - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

JF - Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy

SN - 1386-1425

ER -