Thyroid hormone influences muscle mechanics in carp (Cyprinus carpio) independently from SERCA activity

Rob S. James, Alexander G. Little, Jason Tallis, Frank Seebacher

Research output: Contribution to journalArticle

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Abstract

Thyroid hormone is a key regulator of metabolism, and in zebrafish hypothyroidism decreases sustained and burst swimming performance. These effects are accompanied by decreases in both metabolic scope and the activity of sarco-endoplasmic reticulum ATPase (SERCA) in zebrafish. Our aim was to determine whether thyroid hormone affects skeletal muscle contractile function directly and whether these effects are mediated by influencing SERCA activity. We show that hypothyroidism reduces sustained locomotor performance but not sprint performance in carp (Cyprinus carpio). We accept our hypothesis that hypothyroidism reduces force production in isolated skeletal muscle, when compared to T2, but we reject the hypothesis that this effect is mediated by influencing SERCA activity. Blocking SERCA activity with thapsigargin reduced muscle fatigue resistance, but hypothyroidism had no effect on fatigue. Hence, thyroid hormone plays a role in determining isolated skeletal muscle mechanics, but its effects are more likely to be mediated by other mechanisms than affecting SERCA activity.
Original languageEnglish
Pages (from-to)2806-2808
Number of pages3
JournalJournal of Experimental Biology
Volume219
Early online date8 Jul 2016
DOIs
Publication statusPublished - 21 Sep 2016

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Carps
thyroid hormones
Mechanics
Thyroid Hormones
Cyprinus carpio
mechanics
carp
Endoplasmic Reticulum
hypothyroidism
endoplasmic reticulum
hormone
adenosinetriphosphatase
Adenosine Triphosphatases
Hypothyroidism
muscle
Muscles
muscles
skeletal muscle
Skeletal Muscle
Zebrafish

Keywords

  • Calcium
  • Fatigue
  • Force
  • Hypothyroidism
  • Stress

Cite this

Thyroid hormone influences muscle mechanics in carp (Cyprinus carpio) independently from SERCA activity. / James, Rob S.; Little, Alexander G.; Tallis, Jason; Seebacher, Frank.

In: Journal of Experimental Biology, Vol. 219, 21.09.2016, p. 2806-2808.

Research output: Contribution to journalArticle

James, Rob S. ; Little, Alexander G. ; Tallis, Jason ; Seebacher, Frank. / Thyroid hormone influences muscle mechanics in carp (Cyprinus carpio) independently from SERCA activity. In: Journal of Experimental Biology. 2016 ; Vol. 219. pp. 2806-2808.
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