The thermodynamic landscape of methanogenic PAH degradation

Jan Dolfing, Aiping Xu, Neil Gray, Stephen Larter, Ian Head

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Abstract

Methanogenic degradation of polycyclic aromatic hydrocarbons (PAHs) has long been considered impossible, but evidence in contaminated near surface environments and biodegrading petroleum reservoirs suggests that this is not necessarily the case. To evaluate the thermodynamic constraints on methanogenic PAH degradation we have estimated the Gibbs free energy values for naphthalene, phenanthrene, anthracene, pyrene and chrysene in the aqueous phase, and used these values to evaluate several possible routes whereby PAHs may be converted to methane. Under standard conditions (25°C, solutes at 1 M concentrations, and gases at 1 atm), methanogenic degradation of these PAHs yields between 209 and 331 kJ mol−1. Per mole of methane produced this is 27–35 kJ mol−1, indicating that PAH‐based methanogenesis is exergonic. We evaluated the energetics of three potential PAH degradation routes: oxidation to H2/CO2, complete conversion to acetate, or incomplete oxidation to H2 plus acetate. Depending on the in situ conditions the energetically most favourable pathway for the PAH‐degrading organisms is oxidation to H2/CO2 or conversion into acetate. These are not necessarily the pathways that prevail in the environment. This may be because the kinetic theory of optimal length of metabolic pathways suggests that PAH degraders may have evolved towards incomplete oxidation to acetate plus H2 as the optimal pathway.
Original languageEnglish
Pages (from-to)566-574
Number of pages9
JournalMicrobial Biotechnology
Volume2
Issue number5
DOIs
Publication statusPublished - 21 Aug 2009
Externally publishedYes

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PAH
thermodynamics
degradation
acetate
oxidation
methane
Gibbs free energy
methanogenesis
phenanthrene
naphthalene
pyrene
solute
energetics
petroleum
kinetics
gas

Bibliographical note

This article is open access Creative Commons Attribution License

Cite this

The thermodynamic landscape of methanogenic PAH degradation. / Dolfing, Jan; Xu, Aiping; Gray, Neil; Larter, Stephen; Head, Ian.

In: Microbial Biotechnology, Vol. 2, No. 5, 21.08.2009, p. 566-574.

Research output: Contribution to journalArticle

Dolfing, Jan ; Xu, Aiping ; Gray, Neil ; Larter, Stephen ; Head, Ian. / The thermodynamic landscape of methanogenic PAH degradation. In: Microbial Biotechnology. 2009 ; Vol. 2, No. 5. pp. 566-574.
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