The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles

Messa Guy, Matthw Piasecki, Joshua Hurst, Cameron Hill, Jason Tallis, Hans Degens

Research output: Contribution to journalArticle

Abstract

Prolonged high-fat diets (HFD) can cause intramyocellular lipids (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether effects are muscle-specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles from female CD-1 mice divided into 5 groups: young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-CON, n=28). The young animals were 20 weeks old at the end of the experiment. Sixty 70-week-old female CD-1 mice received either a normal diet (O-CON, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and IMCL content increased in old OS-HFD (p≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (p≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger compared to Y-CON (p≤0.004) while old mice had a larger soleus FCSA compared to CON after only 9 weeks on a HFD (p<0.001). The FCSA of the EDL muscle did not differ significantly between groups. Oxidative capacity of fibres increased in young only, irrespective of HFD duration (p<0.001). High-fat diet-induced morphological changes occur earlier in the old animals when compared to young, and adaptations to HFD are muscle-specific with the EDL being least responsive.
Original languageEnglish
Pages (from-to)(In-press)
JournalJournal of Experimental Biology
Volume(In-press)
Early online date27 Jan 2020
DOIs
Publication statusE-pub ahead of print - 27 Jan 2020

Fingerprint

High Fat Diet
high fat diet
fat
muscle
diet
Muscles
muscles
duration
mice
dietary fiber
diaphragm
Diaphragm
body mass
lipid
Lipids
young animals
animal
young
body mass index

Cite this

The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles. / Guy, Messa; Piasecki, Matthw; Hurst, Joshua; Hill, Cameron; Tallis, Jason; Degens, Hans .

In: Journal of Experimental Biology, Vol. (In-press), 27.01.2020, p. (In-press).

Research output: Contribution to journalArticle

Guy, Messa ; Piasecki, Matthw ; Hurst, Joshua ; Hill, Cameron ; Tallis, Jason ; Degens, Hans . / The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles. In: Journal of Experimental Biology. 2020 ; Vol. (In-press). pp. (In-press).
@article{e0adb80254ba4643883a543545aad1d2,
title = "The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles",
abstract = "Prolonged high-fat diets (HFD) can cause intramyocellular lipids (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether effects are muscle-specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles from female CD-1 mice divided into 5 groups: young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-CON, n=28). The young animals were 20 weeks old at the end of the experiment. Sixty 70-week-old female CD-1 mice received either a normal diet (O-CON, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and IMCL content increased in old OS-HFD (p≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (p≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger compared to Y-CON (p≤0.004) while old mice had a larger soleus FCSA compared to CON after only 9 weeks on a HFD (p<0.001). The FCSA of the EDL muscle did not differ significantly between groups. Oxidative capacity of fibres increased in young only, irrespective of HFD duration (p<0.001). High-fat diet-induced morphological changes occur earlier in the old animals when compared to young, and adaptations to HFD are muscle-specific with the EDL being least responsive.",
author = "Messa Guy and Matthw Piasecki and Joshua Hurst and Cameron Hill and Jason Tallis and Hans Degens",
year = "2020",
month = "1",
day = "27",
doi = "10.1242/jeb.217117",
language = "English",
volume = "(In-press)",
pages = "(In--press)",
journal = "Journal of Experimental Biology",
issn = "0022-0949",
publisher = "Company of Biologists",

}

TY - JOUR

T1 - The impact of a high-fat diet in mice is dependent on duration and age, and differs between muscles

AU - Guy, Messa

AU - Piasecki, Matthw

AU - Hurst, Joshua

AU - Hill, Cameron

AU - Tallis, Jason

AU - Degens, Hans

PY - 2020/1/27

Y1 - 2020/1/27

N2 - Prolonged high-fat diets (HFD) can cause intramyocellular lipids (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether effects are muscle-specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles from female CD-1 mice divided into 5 groups: young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-CON, n=28). The young animals were 20 weeks old at the end of the experiment. Sixty 70-week-old female CD-1 mice received either a normal diet (O-CON, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and IMCL content increased in old OS-HFD (p≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (p≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger compared to Y-CON (p≤0.004) while old mice had a larger soleus FCSA compared to CON after only 9 weeks on a HFD (p<0.001). The FCSA of the EDL muscle did not differ significantly between groups. Oxidative capacity of fibres increased in young only, irrespective of HFD duration (p<0.001). High-fat diet-induced morphological changes occur earlier in the old animals when compared to young, and adaptations to HFD are muscle-specific with the EDL being least responsive.

AB - Prolonged high-fat diets (HFD) can cause intramyocellular lipids (IMCL) accumulation that may negatively affect muscle function. We investigated the duration of a HFD required to instigate these changes, and whether effects are muscle-specific and aggravated in older age. Muscle morphology was determined in the soleus, extensor digitorum longus (EDL) and diaphragm muscles from female CD-1 mice divided into 5 groups: young fed a HFD for 8 weeks (YS-HFD, n=16), young fed a HFD for 16 weeks (YL-HFD, n=28) and young control (Y-CON, n=28). The young animals were 20 weeks old at the end of the experiment. Sixty 70-week-old female CD-1 mice received either a normal diet (O-CON, n=30) or a HFD for 9 weeks (OS-HFD, n=30). Body mass, body mass index and IMCL content increased in old OS-HFD (p≤0.003). In the young mice, this increase was seen in YL-HFD and not YS-HFD (p≤0.006). The soleus and diaphragm fibre cross-sectional area (FCSA) in YL-HFD was larger compared to Y-CON (p≤0.004) while old mice had a larger soleus FCSA compared to CON after only 9 weeks on a HFD (p<0.001). The FCSA of the EDL muscle did not differ significantly between groups. Oxidative capacity of fibres increased in young only, irrespective of HFD duration (p<0.001). High-fat diet-induced morphological changes occur earlier in the old animals when compared to young, and adaptations to HFD are muscle-specific with the EDL being least responsive.

U2 - 10.1242/jeb.217117

DO - 10.1242/jeb.217117

M3 - Article

VL - (In-press)

SP - (In-press)

JO - Journal of Experimental Biology

JF - Journal of Experimental Biology

SN - 0022-0949

ER -