The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Heather J. Sears; Stuart J. Ferguson; David J. Richardson; Stephen Spiro

doi:10.1111/j.1574-6968.1993.tb06496.x

The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Heather J. Sears, Stuart J. Ferguson, David J. Richardson, Stephen Spiro

Research output: Contribution to journal › Article › peer-review

4 Citations (Scopus)

Abstract

A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.

Original language	English
Pages (from-to)	107-111
Number of pages	5
Journal	FEMS Microbiology Letters
Volume	113
Issue number	1
DOIs	https://doi.org/10.1111/j.1574-6968.1993.tb06496.x
Publication status	Published - 1 Oct 1993
Externally published	Yes

Funding

We are gratefult o N. Harms who provided PD1222 and V. Price for assistancwe ith translation of \[17\]f rom French. This work was supported by the Sciencea nd EngineeringC ouncil througha researchs tudentshitpo H.J.S., AFRC Grant PG83/554 to D.J.R. and S.S. and SERC Grant GR/H12225 to D.J.R. and S.J.F.

Keywords

Aerobic denitrification
Nitrate reductase
Paracoccus denitrificans
Periplasm

ASJC Scopus subject areas

Microbiology
Molecular Biology
Genetics

Access to Document

10.1111/j.1574-6968.1993.tb06496.x

Cite this

@article{dcfffdb7b191476d88d363c2cc718538,

title = "The identification of a periplasmic nitrate reductase in Paracoccus denitrificans",

abstract = "A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.",

keywords = "Aerobic denitrification, Nitrate reductase, Paracoccus denitrificans, Periplasm",

author = "Sears, \{Heather J.\} and Ferguson, \{Stuart J.\} and Richardson, \{David J.\} and Stephen Spiro",

year = "1993",

month = oct,

day = "1",

doi = "10.1111/j.1574-6968.1993.tb06496.x",

language = "English",

volume = "113",

pages = "107--111",

journal = "FEMS Microbiology Letters",

issn = "0378-1097",

publisher = "Oxford University Press",

number = "1",

}

TY - JOUR

T1 - The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

AU - Sears, Heather J.

AU - Ferguson, Stuart J.

AU - Richardson, David J.

AU - Spiro, Stephen

PY - 1993/10/1

Y1 - 1993/10/1

N2 - A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.

AB - A nitrate reductase activity has been identified in periplasmic extracts of Paracoccus denitrificans. The enzyme is relatively insensitive to azide and does not reduce chlorate, features which distinguish it from the well-characterised membrane-associated nitrate reductase. The specific activity of the enzyme was higher in intact cells grown with butyrate rather than succinate as the sole source of carbon.

KW - Aerobic denitrification

KW - Nitrate reductase

KW - Paracoccus denitrificans

KW - Periplasm

UR - https://www.scopus.com/pages/publications/85046521395

U2 - 10.1111/j.1574-6968.1993.tb06496.x

DO - 10.1111/j.1574-6968.1993.tb06496.x

M3 - Article

AN - SCOPUS:85046521395

SN - 0378-1097

VL - 113

SP - 107

EP - 111

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

IS - 1

ER -

The identification of a periplasmic nitrate reductase in Paracoccus denitrificans

Abstract

Funding

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this