The expression of the molecular chaperone calnexin is decreased in cancer cells grown as colonies compared to monolayer

LC Yeates, G Powis

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Abstract

Differential display was used to identify genes which were differentially expressed when HT-29 human colon adenocarcinoma cells were grown as monolayers attached to plastic or as colonies in soft agarose. One of the gel bands differentially displayed corresponded to a 171 bp fragment that showed 99% identity with a sequence of mRNA for human calnexin. The decrease in calnexin gene expression by HT-29 cells growing as colonies in soft agarose was confirmed by reverse transcriptase-PCR (RT-PCR) using calnexin-specific primers. We also used RT-PCR to show that the expression of calnexin was decreased in HT-29 cells and MCF-7 human breast adenocarcinoma cells growing in suspension in poly(hydroxyethyl methacrylate)-coated wells compared to cells growing as monolayers. The results suggest that there is a signal for the up-regulation of calnexin expression when cells contact a substrate which allows cell adhesion.
Original languageEnglish
Pages (from-to)66-70
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume238
DOIs
Publication statusPublished - 8 Sep 1997
Externally publishedYes

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