The expression, activity and localisation of the secretory pathway Ca 2+-ATPase (SPCA1) in different mammalian tissues

Laura L. Wootton; Cymone C.H. Argent; Mark Wheatley; Francesco Michelangeli

doi:10.1016/j.bbamem.2004.05.009

The expression, activity and localisation of the secretory pathway Ca ²⁺-ATPase (SPCA1) in different mammalian tissues

Laura L. Wootton
, Cymone C.H. Argent
, Mark Wheatley
, Francesco Michelangeli

University of Birmingham

Research output: Contribution to journal › Article › peer-review

58 Citations (Scopus)

Abstract

The distribution of the secretory pathway Ca²⁺-ATPase (SPCA1) was investigated at both the mRNA and protein level in a variety of tissues. The mRNA and the protein for SPCA1 were relatively abundant in rat brain, testis and testicular derived cells (myoid cells, germ cells, primary Sertoli cells and TM4 cells; a mouse Sertoli cell line) and epididymal fat pads. Lower levels were found in aorta (rat and porcine), heart, liver, lung and kidney. SPCA activities from a number of tissues were measured and shown to be particularly high in brain, aorta, heart, fat pads and testis. As the proportion of SPCA activity compared to total Ca²⁺ ATPase activity in brain, aorta, fat pads and testis were relatively high, this suggests that SPCA1 plays a major role in Ca²⁺ storage within these tissues. The subcellular localisation of SPCA1 was shown to be predominantly around the Golgi in both human aortic smooth muscle cells and TM4 cells.

Original language	English
Pages (from-to)	189-197
Number of pages	9
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1664
Issue number	2
DOIs	https://doi.org/10.1016/j.bbamem.2004.05.009
Publication status	Published - 30 Aug 2004
Externally published	Yes

Funding

We would like to thank the British Heart Foundation for a PhD studentship to L.L.W. and a project grant to M.W (BHF grant number PG/03/082/15735). Marukh Akhtar is thanked for preliminary experiments. We would also like to thank Dr. J.M. East from the University of Southampton, UK for the gift of Y1F4 anti-SERCA antibody. Frances Turner and Dr. N. Hotchin (School of Biosciences, University of Birmingham) are also thanked for advice on immunofluorescence and use of the fluorescence microscope. Prof. Ludwig Missiaen is also thanked for helpful discussions.

Keywords

Activity
Immunofluorescence
RT-PCR
SERCA
SPCA
Western blotting

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/j.bbamem.2004.05.009

Cite this

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title = "The expression, activity and localisation of the secretory pathway Ca 2+-ATPase (SPCA1) in different mammalian tissues",

abstract = "The distribution of the secretory pathway Ca2+-ATPase (SPCA1) was investigated at both the mRNA and protein level in a variety of tissues. The mRNA and the protein for SPCA1 were relatively abundant in rat brain, testis and testicular derived cells (myoid cells, germ cells, primary Sertoli cells and TM4 cells; a mouse Sertoli cell line) and epididymal fat pads. Lower levels were found in aorta (rat and porcine), heart, liver, lung and kidney. SPCA activities from a number of tissues were measured and shown to be particularly high in brain, aorta, heart, fat pads and testis. As the proportion of SPCA activity compared to total Ca2+ ATPase activity in brain, aorta, fat pads and testis were relatively high, this suggests that SPCA1 plays a major role in Ca2+ storage within these tissues. The subcellular localisation of SPCA1 was shown to be predominantly around the Golgi in both human aortic smooth muscle cells and TM4 cells.",

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AU - Michelangeli, Francesco

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AB - The distribution of the secretory pathway Ca2+-ATPase (SPCA1) was investigated at both the mRNA and protein level in a variety of tissues. The mRNA and the protein for SPCA1 were relatively abundant in rat brain, testis and testicular derived cells (myoid cells, germ cells, primary Sertoli cells and TM4 cells; a mouse Sertoli cell line) and epididymal fat pads. Lower levels were found in aorta (rat and porcine), heart, liver, lung and kidney. SPCA activities from a number of tissues were measured and shown to be particularly high in brain, aorta, heart, fat pads and testis. As the proportion of SPCA activity compared to total Ca2+ ATPase activity in brain, aorta, fat pads and testis were relatively high, this suggests that SPCA1 plays a major role in Ca2+ storage within these tissues. The subcellular localisation of SPCA1 was shown to be predominantly around the Golgi in both human aortic smooth muscle cells and TM4 cells.

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