The expression, activity and localisation of the secretory pathway Ca 2+-ATPase (SPCA1) in different mammalian tissues

Laura L. Wootton, Cymone C.H. Argent, Mark Wheatley, Francesco Michelangeli

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

The distribution of the secretory pathway Ca2+-ATPase (SPCA1) was investigated at both the mRNA and protein level in a variety of tissues. The mRNA and the protein for SPCA1 were relatively abundant in rat brain, testis and testicular derived cells (myoid cells, germ cells, primary Sertoli cells and TM4 cells; a mouse Sertoli cell line) and epididymal fat pads. Lower levels were found in aorta (rat and porcine), heart, liver, lung and kidney. SPCA activities from a number of tissues were measured and shown to be particularly high in brain, aorta, heart, fat pads and testis. As the proportion of SPCA activity compared to total Ca2+ ATPase activity in brain, aorta, fat pads and testis were relatively high, this suggests that SPCA1 plays a major role in Ca2+ storage within these tissues. The subcellular localisation of SPCA1 was shown to be predominantly around the Golgi in both human aortic smooth muscle cells and TM4 cells.

Original languageEnglish
Pages (from-to)189-197
Number of pages9
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1664
Issue number2
DOIs
Publication statusPublished - 30 Aug 2004
Externally publishedYes

Keywords

  • Activity
  • Immunofluorescence
  • RT-PCR
  • SERCA
  • SPCA
  • Western blotting

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Cell Biology

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