The Effects of High-Protein Diet and Resistance Training on Glucose Control and Inflammatory Profile of Visceral Adipose Tissue in Rats

Cláudia Stela Medeiros, Ivo Vieira de Sousa Neto, Keemilyn Karla Santos Silva, Ana Paula Castro Cantuária, Taia Maria Berto Rezende, Octávio Luiz Franco, Rita de Cassia Marqueti, Leandro Ceotto Freitas-Lima, Ronaldo Carvalho Araujo, Azize Yildirim, Richard Mackenzie, Jeeser Alves Almeida

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3 Citations (Scopus)
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High-protein diets (HPDs) are widely accepted as a way to stimulate muscle protein synthesis when combined with resistance training (RT). However, the effects of HPDs on adipose tissue plasticity and local inflammation are yet to be determined. This study investigated the impact of HPDs on glucose control, adipocyte size, and epididymal adipose inflammatory biomarkers in resistance-trained rats. Eighteen Wistar rats were randomly assigned to four groups: normal-protein (NPD; 17% protein total dietary intake) and HPD (26.1% protein) without RT and NPD and HPD with RT. Trained groups received RT for 12 weeks with weights secured to their tails. Glucose and insulin tolerance tests, adipocyte size, and an array of cytokines were determined. While HPD without RT induced glucose intolerance, enlarged adipocytes, and increased TNF-α, MCP-1, and IL1-β levels in epididymal adipose tissue (p < 0.05), RT diminished these deleterious effects, with the HPD + RT group displaying improved blood glucose control without inflammatory cytokine increases in epididymal adipose tissue (p < 0.05). Furthermore, RT increased glutathione expression independent of diet (p < 0.05). RT may offer protection against adipocyte hypertrophy, pro-inflammatory states, and glucose intolerance during HPDs. The results highlight the potential protective effects of RT to mitigate the maladaptive effects of HPDs.

Original languageEnglish
Article number1969
Number of pages13
Issue number6
Publication statusPublished - 8 Jun 2021
Externally publishedYes

Bibliographical note

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (


This study was financed in part by the Fundação Universidade Federal de Mato Grosso do Sul—UFMS/MEC—Brazil and in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil (CAPES)—Finance Code 001. The authors are also grateful for the financial support provided by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Universal 408534/2016-8).


  • dietary management
  • exercise training
  • metabolism
  • adipokines


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