The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction

Richard A. Ferguson, Julie E.A. Hunt, Mark P. Lewis, Neil R.W. Martin, Darren J. Player, Carolin Stangier, Conor W. Taylor, Mark C. Turner

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

This study investigated protein kinase activation and gene expression of angiogenic factors in response to low-load resistance exercise with or without blood flow restriction (BFR). In a repeated measures cross-over design, six males performed four sets of bilateral knee extension exercise at 20% 1RM (reps per set = 30:15:15:continued to fatigue) with BFR (110 mmHg) and without (CON). Muscle biopsies were obtained from the vastus lateralis before, 2 and 4 h post-exercise. mRNA expression was determined using real-time RT–PCR. Protein phosphorylation/expression was determined using Western blot. p38MAPK phosphorylation was greater (p = 0.05) at 2 h following BFR (1.3 ± 0.8) compared to CON (0.4 ± 0.3). AMPK phosphorylation remained unchanged. PGC-1α mRNA expression increased at 2 h (5.9 ± 1.3 vs. 2.1 ± 0.8; p = 0.03) and 4 h (3.2 ± 0.8 vs. 1.5 ± 0.4; p = 0.03) following BFR exercise with no change in CON. PGC-1α protein expression did not change following either exercise. BFR exercise enhanced mRNA expression of vascular endothelial growth factor (VEGF) at 2 h (5.2 ± 2.8 vs 1.7 ± 1.1; p =.02) and 4 h (6.8 ± 4.9 vs. 2.5 ± 2.7; p =.01) compared to CON. mRNA expression of VEGF-R2 and hypoxia-inducible factor 1α increased following BFR exercise but only eNOS were enhanced relative to CON. Matrix metalloproteinase-9 mRNA expression was not altered in response to either exercise. Acute low-load resistance exercise with BFR provides a targeted angiogenic response potentially mediated through enhanced ischaemic and shear stress stimuli.

Original languageEnglish
Pages (from-to)397-406
Number of pages10
JournalEuropean Journal of Sport Science
Volume18
Issue number3
Early online date17 Jan 2018
DOIs
Publication statusPublished - 16 Mar 2018
Externally publishedYes

Fingerprint

Messenger RNA
Phosphorylation
Vascular Endothelial Growth Factor A
Hypoxia-Inducible Factor 1
AMP-Activated Protein Kinases
Angiogenesis Inducing Agents
Matrix Metalloproteinase 9
Quadriceps Muscle
Cross-Over Studies
Protein Kinases
Fatigue
Knee
Proteins
Western Blotting
Biopsy
Gene Expression
Muscles

Keywords

  • BFR
  • capillaries
  • hypoxia
  • kaatsu
  • resistance training

ASJC Scopus subject areas

  • Physical Therapy, Sports Therapy and Rehabilitation
  • Orthopedics and Sports Medicine

Cite this

The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction. / Ferguson, Richard A.; Hunt, Julie E.A.; Lewis, Mark P.; Martin, Neil R.W.; Player, Darren J.; Stangier, Carolin; Taylor, Conor W.; Turner, Mark C.

In: European Journal of Sport Science, Vol. 18, No. 3, 16.03.2018, p. 397-406.

Research output: Contribution to journalArticle

Ferguson, Richard A. ; Hunt, Julie E.A. ; Lewis, Mark P. ; Martin, Neil R.W. ; Player, Darren J. ; Stangier, Carolin ; Taylor, Conor W. ; Turner, Mark C. / The acute angiogenic signalling response to low-load resistance exercise with blood flow restriction. In: European Journal of Sport Science. 2018 ; Vol. 18, No. 3. pp. 397-406.
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