Substrate selectivity in the zDHHC family of S-acyltransferases

Kimon Lemonidis, Christine Salaun, Marianna Kouskou, Cinta Diez-Ardanuy, Luke H. Chamberlain, Jennifer Greaves

Research output: Contribution to journalReview article

7 Citations (Scopus)

Abstract

S-acylation is a reversible lipid modification occurring on cysteine residues mediated by a family of membrane-bound 'zDHHC' enzymes. S-acylation predominantly results in anchoring of soluble proteins to membrane compartments or in the trafficking of membrane proteins to different compartments. Recent work has shown that although S-acylation of some proteins may involve very weak interactions with zDHHC enzymes, a pool of zDHHC enzymes exhibit strong and specific interactions with substrates, thereby recruiting them for S-acylation. For example, the ankyrin-repeat domains of zDHHC17 and zDHHC13 interact specifically with unstructured consensus sequences present in some proteins, thus contributing to substrate specificity of these enzymes. In addition to this new information on zDHHC enzyme protein substrate specificity, recent work has also identified marked differences in selectivity of zDHHC enzymes for acyl-CoA substrates and has started to unravel the underlying molecular basis for this lipid selectivity. This review will focus on the protein and acyl-CoA selectivity of zDHHC enzymes.

Original languageEnglish
Pages (from-to)751-758
Number of pages8
JournalBiochemical Society Transactions
Volume45
Issue number3
DOIs
Publication statusPublished - 15 Jun 2017
Externally publishedYes

Fingerprint

Acyltransferases
Acylation
Substrates
Enzymes
Acyl Coenzyme A
Substrate Specificity
Proteins
Membrane Proteins
Ankyrin Repeat
Membranes
Lipids
Consensus Sequence
Cysteine

Keywords

  • palmitoylation
  • S-acylation
  • S-palmitoylation
  • zDHHC enzymes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Lemonidis, K., Salaun, C., Kouskou, M., Diez-Ardanuy, C., Chamberlain, L. H., & Greaves, J. (2017). Substrate selectivity in the zDHHC family of S-acyltransferases. Biochemical Society Transactions, 45(3), 751-758. https://doi.org/10.1042/BST20160309

Substrate selectivity in the zDHHC family of S-acyltransferases. / Lemonidis, Kimon; Salaun, Christine; Kouskou, Marianna; Diez-Ardanuy, Cinta; Chamberlain, Luke H.; Greaves, Jennifer.

In: Biochemical Society Transactions, Vol. 45, No. 3, 15.06.2017, p. 751-758.

Research output: Contribution to journalReview article

Lemonidis, K, Salaun, C, Kouskou, M, Diez-Ardanuy, C, Chamberlain, LH & Greaves, J 2017, 'Substrate selectivity in the zDHHC family of S-acyltransferases' Biochemical Society Transactions, vol. 45, no. 3, pp. 751-758. https://doi.org/10.1042/BST20160309
Lemonidis K, Salaun C, Kouskou M, Diez-Ardanuy C, Chamberlain LH, Greaves J. Substrate selectivity in the zDHHC family of S-acyltransferases. Biochemical Society Transactions. 2017 Jun 15;45(3):751-758. https://doi.org/10.1042/BST20160309
Lemonidis, Kimon ; Salaun, Christine ; Kouskou, Marianna ; Diez-Ardanuy, Cinta ; Chamberlain, Luke H. ; Greaves, Jennifer. / Substrate selectivity in the zDHHC family of S-acyltransferases. In: Biochemical Society Transactions. 2017 ; Vol. 45, No. 3. pp. 751-758.
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