Study on Theileria lestoquardi antigens as potential vaccine candidates

Mehdi Namavari; Mohammad H. Hosseini; Atefeh Seghatoleslam; Mohsen Lotfi; Ali Shirazi; Olivier A.E. Sparagano

doi:10.1196/annals.1428.020

Study on Theileria lestoquardi antigens as potential vaccine candidates

Mehdi Namavari
, Mohammad H. Hosseini
, Atefeh Seghatoleslam
, Mohsen Lotfi
, Ali Shirazi
, Olivier A.E. Sparagano

Razi Vaccine and Serum Research Institute
Shiraz University of Medical Sciences
Newcastle University

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding › peer-review

2 Citations (Scopus)

Abstract

Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.

Original language	English
Title of host publication	Animal Biodiversity and Emerging Diseases Prediction and Prevention
Publisher	Wiley
Pages	205-207
Number of pages	3
Volume	1149
ISBN (Print)	9781573317146
DOIs	https://doi.org/10.1196/annals.1428.020
Publication status	Published - 15 Dec 2008
Externally published	Yes

Publication series

Name	Annals of the New York Academy of Sciences
Volume	1149
ISSN (Print)	0077-8923
ISSN (Electronic)	1749-6632

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Antigen
Schizont
Sheep
Theileria lestoquardi

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
History and Philosophy of Science

Access to Document

10.1196/annals.1428.020

Cite this

Namavari, M., Hosseini, M. H., Seghatoleslam, A., Lotfi, M., Shirazi, A., & Sparagano, O. A. E. (2008). Study on Theileria lestoquardi antigens as potential vaccine candidates. In Animal Biodiversity and Emerging Diseases Prediction and Prevention (Vol. 1149, pp. 205-207). (Annals of the New York Academy of Sciences; Vol. 1149). Wiley. https://doi.org/10.1196/annals.1428.020

Study on Theileria lestoquardi antigens as potential vaccine candidates. / Namavari, Mehdi; Hosseini, Mohammad H.; Seghatoleslam, Atefeh et al.
Animal Biodiversity and Emerging Diseases Prediction and Prevention. Vol. 1149 Wiley, 2008. p. 205-207 (Annals of the New York Academy of Sciences; Vol. 1149).

Research output: Chapter in Book/Report/Conference proceeding › Conference proceeding › peer-review

@inproceedings{b27cf6e74e1a403abcb1c8aa012126e6,

title = "Study on Theileria lestoquardi antigens as potential vaccine candidates",

abstract = "Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.",

keywords = "Antigen, Schizont, Sheep, Theileria lestoquardi",

author = "Mehdi Namavari and Hosseini, \{Mohammad H.\} and Atefeh Seghatoleslam and Mohsen Lotfi and Ali Shirazi and Sparagano, \{Olivier A.E.\}",

year = "2008",

month = dec,

day = "15",

doi = "10.1196/annals.1428.020",

language = "English",

isbn = "9781573317146",

volume = "1149",

series = "Annals of the New York Academy of Sciences",

publisher = "Wiley",

pages = "205--207",

booktitle = "Animal Biodiversity and Emerging Diseases Prediction and Prevention",

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}

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T1 - Study on Theileria lestoquardi antigens as potential vaccine candidates

AU - Namavari, Mehdi

AU - Hosseini, Mohammad H.

AU - Seghatoleslam, Atefeh

AU - Lotfi, Mohsen

AU - Shirazi, Ali

AU - Sparagano, Olivier A.E.

PY - 2008/12/15

Y1 - 2008/12/15

N2 - Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.

AB - Theileria lestoquardi is the causative agent of malignant theileriosis of sheep and goats, causing morbidity and mortality in these animals worldwide. Western blot analysis based on T. lestoquardi schizont antigens was carried out using sera collected from Iranian sheep, which had been immunized with T. lestoquardi schizont-infected cells. The results of Western blot analysis demonstrated that schizont-immunized animals produced antibodies reacting with protein bands at 73, 42, 20, 14, and 12 kDa. Comparison of the results of the current Western blotting test with earlier studies of Theileria spp. revealed two immunogenic schizont proteins with molecular weights of 73 and 42 kDa shared between T. annulata and T. lestoquardi. Two other proteins with molecular weights of 14 and 12 kDa have not been previously found in other Theileria species. Our results suggest that the 73-kDa protein could be a potential vaccine candidate and that the 14- and 12-kDa proteins could be considered as diagnostic antigens.

KW - Antigen

KW - Schizont

KW - Sheep

KW - Theileria lestoquardi

UR - https://www.scopus.com/pages/publications/57849131924

U2 - 10.1196/annals.1428.020

DO - 10.1196/annals.1428.020

M3 - Conference proceeding

C2 - 19120211

AN - SCOPUS:57849131924

SN - 9781573317146

VL - 1149

T3 - Annals of the New York Academy of Sciences

SP - 205

EP - 207

BT - Animal Biodiversity and Emerging Diseases Prediction and Prevention

PB - Wiley

ER -

Study on Theileria lestoquardi antigens as potential vaccine candidates

Abstract

Publication series

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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