Structure and dynamics of the active Gs-coupled human secretin receptor

Maoqing Dong; Giuseppe Deganutti; Sarah J. Piper; Yi Lynn Liang; Maryam Khoshouei; Matthew J. Belousoff; Kaleeckal G. Harikumar; Christopher A. Reynolds; Alisa Glukhova; Sebastian G.B. Furness; Arthur Christopoulos; Radostin Danev; Denise Wootten; Patrick M. Sexton; Laurence J. Miller

doi:10.1038/s41467-020-17791-4

Structure and dynamics of the active Gs-coupled human secretin receptor

Maoqing Dong, Giuseppe Deganutti, Sarah J. Piper, Yi Lynn Liang, Maryam Khoshouei, Matthew J. Belousoff, Kaleeckal G. Harikumar, Christopher A. Reynolds, Alisa Glukhova, Sebastian G.B. Furness, Arthur Christopoulos, Radostin Danev, Denise Wootten, Patrick M. Sexton, Laurence J. Miller

Research output: Contribution to journal › Article › peer-review

40 Citations (Scopus)

123 Downloads (Pure)

Abstract

The class B secretin GPCR (SecR) has broad physiological effects, with target potential for treatment of metabolic and cardiovascular disease. Molecular understanding of SecR binding and activation is important for its therapeutic exploitation. We combined cryo-electron microscopy, molecular dynamics, and biochemical cross-linking to determine a 2.3 Å structure, and interrogate dynamics, of secretin bound to the SecR:Gs complex. SecR exhibited a unique organization of its extracellular domain (ECD) relative to its 7-transmembrane (TM) core, forming more extended interactions than other family members. Numerous polar interactions formed between secretin and the receptor extracellular loops (ECLs) and TM helices. Cysteine-cross-linking, cryo-electron microscopy multivariate analysis and molecular dynamics simulations revealed that interactions between peptide and receptor were dynamic, and suggested a model for initial peptide engagement where early interactions between the far N-terminus of the peptide and SecR ECL2 likely occur following initial binding of the peptide C-terminus to the ECD.

Original language	English
Article number	4137
Number of pages	17
Journal	Nature Communications
Volume	11
DOIs	https://doi.org/10.1038/s41467-020-17791-4
Publication status	Published - 18 Aug 2020

Bibliographical note

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.

Funder

The work was supported by a grant from the National Institutes of Health, GM-132095 (L.J.M. and P.M.S.), the Monash University Ramaciotti Centre for Cryo-Electron Microscopy, the Monash MASSIVE high-performance computing facility, the National Health and Medical Research Council of Australia (NHMRC) project grant (1120919), and NHMRC program grant (1150083). P.M.S. and A.C. are NHMRC Senior Principal Research Fellows and D.W. is an NHMRC Senior Research Fellow. C.A.R. holds a Royal Society Industry Fellowship and acknowledges a grant from the United Kingdom Biotechnology and Biological Sciences Research Council (BB/M006883/1). R.D. was supported by Takeda Science Foundation 2019 Medical Research Grant and Japan Science and Technology Agency PRESTO (18069571).

Funding

The work was supported by a grant from the National Institutes of Health, GM-132095 (L.J.M. and P.M.S.), the Monash University Ramaciotti Centre for Cryo-Electron Microscopy, the Monash MASSIVE high-performance computing facility, the National Health and Medical Research Council of Australia (NHMRC) project grant (1120919), and NHMRC program grant (1150083). P.M.S. and A.C. are NHMRC Senior Principal Research Fellows and D.W. is an NHMRC Senior Research Fellow. C.A.R. holds a Royal Society Industry Fellowship and acknowledges a grant from the United Kingdom Biotechnology and Biological Sciences Research Council (BB/M006883/1). R.D. was supported by Takeda Science Foundation 2019 Medical Research Grant and Japan Science and Technology Agency PRESTO (18069571). Model residue interaction analysis. Interactions in the PDB (6WZG), between the chains of the peptide and receptor (P:R) or receptor and G proteins (R:A and R:B), were analyzed using the \u201CDimplot\u201D module within the Ligplot+ program (v2.2)44. Hydrogen bonds were additionally analyzed using the UCSF ChimeraX package, with relaxed distance and angle criteria (0.4 A\u030A and 20\u00B0 tolerance, respectively). Additional analyses and production of images were performed using the UCSF Chimera package (v1.14) from the Computer Graphics Laboratory, University of California, San Francisco (supported by NIH P41 RR-01081) and ChimeraX45 (support from National Institutes of Health R01-GM129325).

Funders	Funder number
Biotechnology and Biological Sciences Research Council	BB/M006883/1
National Institutes of Health	R01GM132095
Takeda Science Foundation
National Health and Medical Research Council	1150083, 1120919
Japan Science and Technology Agency	18069571

Keywords

Cryoelectron microscopy
Gastroenterology
Structural biology

ASJC Scopus subject areas

General Chemistry
General Biochemistry,Genetics and Molecular Biology
General Physics and Astronomy

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1038/s41467-020-17791-4Licence: CC BY

PublishedFinal published version, 4.04 MBLicence: CC BY

Cite this

Dong, M., Deganutti, G., Piper, S. J., Liang, Y. L., Khoshouei, M., Belousoff, M. J., Harikumar, K. G., Reynolds, C. A., Glukhova, A., Furness, S. G. B., Christopoulos, A., Danev, R., Wootten, D., Sexton, P. M., & Miller, L. J. (2020). Structure and dynamics of the active Gs-coupled human secretin receptor. Nature Communications, 11, Article 4137. https://doi.org/10.1038/s41467-020-17791-4

@article{beccde95cd3447378be5bf6444b63597,

title = "Structure and dynamics of the active Gs-coupled human secretin receptor",

abstract = "The class B secretin GPCR (SecR) has broad physiological effects, with target potential for treatment of metabolic and cardiovascular disease. Molecular understanding of SecR binding and activation is important for its therapeutic exploitation. We combined cryo-electron microscopy, molecular dynamics, and biochemical cross-linking to determine a 2.3 {\AA} structure, and interrogate dynamics, of secretin bound to the SecR:Gs complex. SecR exhibited a unique organization of its extracellular domain (ECD) relative to its 7-transmembrane (TM) core, forming more extended interactions than other family members. Numerous polar interactions formed between secretin and the receptor extracellular loops (ECLs) and TM helices. Cysteine-cross-linking, cryo-electron microscopy multivariate analysis and molecular dynamics simulations revealed that interactions between peptide and receptor were dynamic, and suggested a model for initial peptide engagement where early interactions between the far N-terminus of the peptide and SecR ECL2 likely occur following initial binding of the peptide C-terminus to the ECD.",

keywords = "Cryoelectron microscopy, Gastroenterology, Structural biology",

author = "Maoqing Dong and Giuseppe Deganutti and Piper, {Sarah J.} and Liang, {Yi Lynn} and Maryam Khoshouei and Belousoff, {Matthew J.} and Harikumar, {Kaleeckal G.} and Reynolds, {Christopher A.} and Alisa Glukhova and Furness, {Sebastian G.B.} and Arthur Christopoulos and Radostin Danev and Denise Wootten and Sexton, {Patrick M.} and Miller, {Laurence J.}",

note = "Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article{\textquoteright}s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article{\textquoteright}s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.",

year = "2020",

month = aug,

day = "18",

doi = "10.1038/s41467-020-17791-4",

language = "English",

volume = "11",

journal = "Nature Communications",

issn = "2041-1723",

publisher = "Nature Publishing Group",

}

TY - JOUR

T1 - Structure and dynamics of the active Gs-coupled human secretin receptor

AU - Dong, Maoqing

AU - Deganutti, Giuseppe

AU - Piper, Sarah J.

AU - Liang, Yi Lynn

AU - Khoshouei, Maryam

AU - Belousoff, Matthew J.

AU - Harikumar, Kaleeckal G.

AU - Reynolds, Christopher A.

AU - Glukhova, Alisa

AU - Furness, Sebastian G.B.

AU - Christopoulos, Arthur

AU - Danev, Radostin

AU - Wootten, Denise

AU - Sexton, Patrick M.

AU - Miller, Laurence J.

N1 - Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/ licenses/by/4.0/.

PY - 2020/8/18

Y1 - 2020/8/18

N2 - The class B secretin GPCR (SecR) has broad physiological effects, with target potential for treatment of metabolic and cardiovascular disease. Molecular understanding of SecR binding and activation is important for its therapeutic exploitation. We combined cryo-electron microscopy, molecular dynamics, and biochemical cross-linking to determine a 2.3 Å structure, and interrogate dynamics, of secretin bound to the SecR:Gs complex. SecR exhibited a unique organization of its extracellular domain (ECD) relative to its 7-transmembrane (TM) core, forming more extended interactions than other family members. Numerous polar interactions formed between secretin and the receptor extracellular loops (ECLs) and TM helices. Cysteine-cross-linking, cryo-electron microscopy multivariate analysis and molecular dynamics simulations revealed that interactions between peptide and receptor were dynamic, and suggested a model for initial peptide engagement where early interactions between the far N-terminus of the peptide and SecR ECL2 likely occur following initial binding of the peptide C-terminus to the ECD.

AB - The class B secretin GPCR (SecR) has broad physiological effects, with target potential for treatment of metabolic and cardiovascular disease. Molecular understanding of SecR binding and activation is important for its therapeutic exploitation. We combined cryo-electron microscopy, molecular dynamics, and biochemical cross-linking to determine a 2.3 Å structure, and interrogate dynamics, of secretin bound to the SecR:Gs complex. SecR exhibited a unique organization of its extracellular domain (ECD) relative to its 7-transmembrane (TM) core, forming more extended interactions than other family members. Numerous polar interactions formed between secretin and the receptor extracellular loops (ECLs) and TM helices. Cysteine-cross-linking, cryo-electron microscopy multivariate analysis and molecular dynamics simulations revealed that interactions between peptide and receptor were dynamic, and suggested a model for initial peptide engagement where early interactions between the far N-terminus of the peptide and SecR ECL2 likely occur following initial binding of the peptide C-terminus to the ECD.

KW - Cryoelectron microscopy

KW - Gastroenterology

KW - Structural biology

UR - http://www.scopus.com/inward/record.url?scp=85089545766&partnerID=8YFLogxK

U2 - 10.1038/s41467-020-17791-4

DO - 10.1038/s41467-020-17791-4

M3 - Article

C2 - 32811827

AN - SCOPUS:85089545766

SN - 2041-1723

VL - 11

JO - Nature Communications

JF - Nature Communications

M1 - 4137

ER -

Structure and dynamics of the active Gs-coupled human secretin receptor

Abstract

Bibliographical note

Funder

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Giuseppe Deganutti

Chris Reynolds

Cite this

Structure and dynamics of the active Gs-coupled human secretin receptor

Abstract

Bibliographical note

Funder

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

Other files and links

Fingerprint

Profiles

Giuseppe Deganutti

Chris Reynolds

Cite this