Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

Mohammed Jamshad, Vinciane Grimard, Ilaria Idini, Tim J. Knowles, Miriam R. Dowle, Naomi Schofield, Pooja Sridhar, Yupin Lin, Rachael Finka, Mark Wheatley, Owen R.T. Thomas, Richard E. Palmer, Michael Overduin, Cédric Govaerts, Jean Marie Ruysschaert, Karen J. Edler, Tim R. Dafforn

Research output: Contribution to journalArticle

69 Citations (Scopus)

Abstract

In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer (poly(styrene-co-maleic acid), SMA) to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle (SMALP) using data from small angle neutron scattering (SANS), electron microscopy (EM), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FTIR), differential scanning calorimetry (DSC) and nuclear magnetic resonance spectroscopy (NMR). We show that the particle is disc shaped containing a polymer “bracelet” encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.

[Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)774-789
Number of pages16
JournalNano Research
Volume8
Issue number3
Early online date23 Oct 2014
DOIs
Publication statusPublished - 1 Jan 2015
Externally publishedYes

Fingerprint

Lipid bilayers
Detergents
Structural analysis
Styrene
Membrane Proteins
Nanoparticles
Proteins
Membranes
Polymers
Lipids
Acids
Membrane Lipids
Neutron scattering
Electron microscopy
Nuclear magnetic resonance spectroscopy
Differential scanning calorimetry
Fourier transform infrared spectroscopy
styrene-maleic acid polymer

Keywords

  • detergent
  • lipid
  • membrane proteins
  • nanoparticles
  • polymer
  • structure

ASJC Scopus subject areas

  • Materials Science(all)
  • Electrical and Electronic Engineering

Cite this

Jamshad, M., Grimard, V., Idini, I., Knowles, T. J., Dowle, M. R., Schofield, N., ... Dafforn, T. R. (2015). Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. Nano Research, 8(3), 774-789. https://doi.org/10.1007/s12274-014-0560-6

Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. / Jamshad, Mohammed; Grimard, Vinciane; Idini, Ilaria; Knowles, Tim J.; Dowle, Miriam R.; Schofield, Naomi; Sridhar, Pooja; Lin, Yupin; Finka, Rachael; Wheatley, Mark; Thomas, Owen R.T.; Palmer, Richard E.; Overduin, Michael; Govaerts, Cédric; Ruysschaert, Jean Marie; Edler, Karen J.; Dafforn, Tim R.

In: Nano Research, Vol. 8, No. 3, 01.01.2015, p. 774-789.

Research output: Contribution to journalArticle

Jamshad, M, Grimard, V, Idini, I, Knowles, TJ, Dowle, MR, Schofield, N, Sridhar, P, Lin, Y, Finka, R, Wheatley, M, Thomas, ORT, Palmer, RE, Overduin, M, Govaerts, C, Ruysschaert, JM, Edler, KJ & Dafforn, TR 2015, 'Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins' Nano Research, vol. 8, no. 3, pp. 774-789. https://doi.org/10.1007/s12274-014-0560-6
Jamshad, Mohammed ; Grimard, Vinciane ; Idini, Ilaria ; Knowles, Tim J. ; Dowle, Miriam R. ; Schofield, Naomi ; Sridhar, Pooja ; Lin, Yupin ; Finka, Rachael ; Wheatley, Mark ; Thomas, Owen R.T. ; Palmer, Richard E. ; Overduin, Michael ; Govaerts, Cédric ; Ruysschaert, Jean Marie ; Edler, Karen J. ; Dafforn, Tim R. / Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins. In: Nano Research. 2015 ; Vol. 8, No. 3. pp. 774-789.
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