STAT5 signaling in kisspeptin cells regulates the timing of puberty

Marina Augusto Silveira, Isadora C Furigo, Thais T Zampieri, Tabata M Bohlen, Daniella G de Paula, Celso Rodrigues Franci, Jose Donato, Renata Frazao

Research output: Contribution to journalArticlepeer-review

27 Citations (Scopus)

Abstract

Previous studies have shown that kisspeptin neurons are important mediators of prolactin's effects on reproduction. However, the cellular mechanisms recruited by prolactin to affect kisspeptin neurons remain unknown. Using whole-cell patch-clamp recordings of brain slices from kisspeptin reporter mice, we observed that 20% of kisspeptin neurons in the anteroventral periventricular nucleus was indirectly depolarized by prolactin via an unknown population of prolactin responsive neurons. This effect required the phosphatidylinositol 3-kinase signaling pathway. No effects on the activity of arcuate kisspeptin neurons were observed, despite a high percentage (70%) of arcuate neurons expressing prolactin-induced STAT5 phosphorylation. To determine whether STAT5 expression in kisspeptin cells regulates reproduction, mice carrying Stat5a/b inactivation specifically in kisspeptin cells were generated. These mutants exhibited an early onset of estrous cyclicity, indicating that STAT5 transcription factors exert an inhibitory effect on the timing of puberty.

Original languageEnglish
Pages (from-to)55-65
Number of pages11
JournalMolecular and Cellular Endocrinology
Volume448
Early online date23 Mar 2017
DOIs
Publication statusPublished - 15 Jun 2017
Externally publishedYes

Bibliographical note

Copyright © 2017 Elsevier B.V. All rights reserved.

Keywords

  • Prolactin
  • Kiss1
  • Sexual maturation
  • HPG axis
  • Hypothalamus
  • Whole-cell patch-clamp

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