Sphingolipid synthesis and scavenging in the intracellular apicomplexan parasite, Toxoplasma gondii

Steven Pratt, Nilu Wansadhipathi-Kannangara, Catherine Bruce, John Mina, Hosam Shams-Eldin, Josefina Casas, Kentaro Hanada, Ralph Schwarz, Sabrina Sonda, Paul Denny

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Sphingolipids are essential components of eukaryotic cell membranes, particularly the plasma membrane, and are involved in a diverse array of signal transduction pathways. Mammals produce sphingomyelin (SM) as the primary complex sphingolipid via the well characterised SM synthase. In contrast yeast, plants and some protozoa utilise an evolutionarily related inositol phosphorylceramide (IPC) synthase to synthesise IPC. This activity has no mammalian equivalent and IPC synthase has been proposed as a target for anti-fungals and anti-protozoals. However, detailed knowledge of the sphingolipid biosynthetic pathway of the apicomplexan protozoan parasites was lacking. In this study bioinformatic analyses indicated a single copy orthologue of the putative SM synthase from the apicomplexan Plasmodium falciparum (the causative agent of malaria) was a bona fide sphingolipid synthase in the related model parasite, Toxoplasma gondii (TgSLS). Subsequently, TgSLS was indicated, by complementation of a mutant cell line, to be a functional orthologue of the yeast IPC synthase (AUR1p), demonstrating resistance to the well characterised AUR1p inhibitor aureobasidin A. In vitro, recombinant TgSLS exhibited IPC synthase activity and, for the first time, the presence of IPC was demonstrated in T. gondii lipid extracts by mass spectrometry. Furthermore, host sphingolipid biosynthesis was indicated to influence, but be non-essential for, T. gondii proliferation, suggesting that whilst scavenging does take place de novo sphingolipid synthesis may be important for parasitism.
Original languageEnglish
Pages (from-to)43-51
Number of pages9
JournalJournal of Molecular a d Biochemical Parasitology
Volume187
Early online date16 Dec 2012
DOIs
Publication statusPublished - 24 Jan 2013
Externally publishedYes

Bibliographical note

© 2012 Elsevier B.V. Open access under CC BY license.

Keywords

  • ToxoplasmaSphingolipid
  • Inositol phosphorylceramide synthase
  • Host–parasite interaction

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