Spectroscopic characterization of a novel multiheme c-type cytochrome widely implicated in bacterial electron transport

M D Roldán, H J Sears, M R Cheesman, S J Ferguson, A J Thomson, B C Berks, D J Richardson

Research output: Contribution to journalArticle

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Abstract

NapC is a member of a family of bacterial membrane-anchored tetra-heme c-type cytochromes that participate in a number of respiratory electron transport pathways. They are postulated to mediate electron transfer between membrane quinols/quinones and soluble periplasmic enzymes. The water-soluble heme domain of NapC has been expressed as a periplasmic protein. Mediated redox potentiometry and characterization by UV-visible, magnetic circular dichroism, and electron paramagnetic resonance spectroscopies demonstrates that soluble NapC contains four low spin hemes, each with bis-histidine axial ligation and with midpoint reduction potentials of -56, -181, -207, and -235 mV.

Original languageEnglish
Pages (from-to)28785-28790
Number of pages6
JournalJournal of Biological Chemistry
Volume273
Issue number44
DOIs
Publication statusPublished - 30 Oct 1998

Fingerprint

Cytochrome c Group
Electron Transport
Heme
Respiratory Transport
Potentiometry
Periplasmic Proteins
Hydroquinones
Membranes
Quinones
Electron Spin Resonance Spectroscopy
Circular Dichroism
Histidine
Oxidation-Reduction
Ligation
Paramagnetic resonance
Spectrum Analysis
Spectroscopy
Electrons
Water
Enzymes

Keywords

  • Amino Acid Sequence
  • Bacteria
  • Base Sequence
  • Cytochrome c Group
  • DNA Primers
  • Electron Transport
  • Molecular Sequence Data
  • Potentiometry
  • Sequence Homology, Amino Acid
  • Spectrum Analysis
  • Journal Article
  • Research Support, Non-U.S. Gov't

Cite this

Spectroscopic characterization of a novel multiheme c-type cytochrome widely implicated in bacterial electron transport. / Roldán, M D; Sears, H J; Cheesman, M R; Ferguson, S J; Thomson, A J; Berks, B C; Richardson, D J.

In: Journal of Biological Chemistry, Vol. 273, No. 44, 30.10.1998, p. 28785-28790.

Research output: Contribution to journalArticle

Roldán, M D ; Sears, H J ; Cheesman, M R ; Ferguson, S J ; Thomson, A J ; Berks, B C ; Richardson, D J. / Spectroscopic characterization of a novel multiheme c-type cytochrome widely implicated in bacterial electron transport. In: Journal of Biological Chemistry. 1998 ; Vol. 273, No. 44. pp. 28785-28790.
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AU - Berks, B C

AU - Richardson, D J

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KW - Base Sequence

KW - Cytochrome c Group

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KW - Research Support, Non-U.S. Gov't

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