Soil bacterial and fungal communities across a pH gradient in an arable soil

J. Rousk, E. Baath, Philip C. Brookes, C.L. Lauber, C. Lozupone, J. Gregory Caporaso, R. Knight, N. Fierer

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    Abstract

    Soils collected across a long-term liming experiment (pH 4.0–8.3), in which variation in factors other than pH have been minimized, were used to investigate the direct influence of pH on the abundance and composition of the two major soil microbial taxa, fungi and bacteria. We hypothesized that bacterial communities would be more strongly influenced by pH than fungal communities. To determine the relative abundance of bacteria and fungi, we used quantitative PCR (qPCR), and to analyze the composition and diversity of the bacterial and fungal communities, we used a bar-coded pyrosequencing technique. Both the relative abundance and diversity of bacteria were positively related to pH, the latter nearly doubling between pH 4 and 8. In contrast, the relative abundance of fungi was unaffected by pH and fungal diversity was only weakly related with pH. The composition of the bacterial communities was closely defined by soil pH; there was as much variability in bacterial community composition across the 180-m distance of this liming experiment as across soils collected from a wide range of biomes in North and South America, emphasizing the dominance of pH in structuring bacterial communities. The apparent direct influence of pH on bacterial community composition is probably due to the narrow pH ranges for optimal growth of bacteria. Fungal community composition was less strongly affected by pH, which is consistent with pure culture studies, demonstrating that fungi generally exhibit wider pH ranges for optimal growth.
    Original languageEnglish
    Pages (from-to)1340–1351
    JournalISME (International Society of Microbial Ecology) Journal
    Volume4
    Issue number10
    DOIs
    Publication statusPublished - 6 May 2010

    Fingerprint

    Proton-Motive Force
    soil fungi
    soil bacteria
    arable soils
    Soil
    fungus
    community composition
    relative abundance
    bacterium
    liming
    bacterial communities
    soil
    fungal communities
    biome
    Fungi
    Bacteria
    experiment
    fungi
    bacteria
    South America

    Bibliographical note

    Philip Brookes is professor at the Centre of Agro-Ecology and Food Security

    Keywords

    • bacterial community
    • fungal community
    • pyrosequencing
    • quantitative PCR
    • Rothamsted Hoosfield Acid Strip
    • soil pH

    Cite this

    Rousk, J., Baath, E., Brookes, P. C., Lauber, C. L., Lozupone, C., Caporaso, J. G., ... Fierer, N. (2010). Soil bacterial and fungal communities across a pH gradient in an arable soil. ISME (International Society of Microbial Ecology) Journal, 4(10), 1340–1351. https://doi.org/10.1038/ismej.2010.58

    Soil bacterial and fungal communities across a pH gradient in an arable soil. / Rousk, J.; Baath, E.; Brookes, Philip C.; Lauber, C.L.; Lozupone, C.; Caporaso, J. Gregory; Knight, R.; Fierer, N.

    In: ISME (International Society of Microbial Ecology) Journal, Vol. 4, No. 10, 06.05.2010, p. 1340–1351.

    Research output: Contribution to journalArticle

    Rousk, J, Baath, E, Brookes, PC, Lauber, CL, Lozupone, C, Caporaso, JG, Knight, R & Fierer, N 2010, 'Soil bacterial and fungal communities across a pH gradient in an arable soil' ISME (International Society of Microbial Ecology) Journal, vol. 4, no. 10, pp. 1340–1351. https://doi.org/10.1038/ismej.2010.58
    Rousk, J. ; Baath, E. ; Brookes, Philip C. ; Lauber, C.L. ; Lozupone, C. ; Caporaso, J. Gregory ; Knight, R. ; Fierer, N. / Soil bacterial and fungal communities across a pH gradient in an arable soil. In: ISME (International Society of Microbial Ecology) Journal. 2010 ; Vol. 4, No. 10. pp. 1340–1351.
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