Short-term creatine supplementation decreases reactive oxygen species content with no changes in expression and activity of antioxidant enzymes in skeletal muscle

Lucas Guimarães-Ferreira, Carlos Hermano J Pinheiro, Frederico Gerlinger-Romero, Kaio Fernando Vitzel, Renato T Nachbar, Rui Curi, Maria Tereza Nunes

Research output: Contribution to journalArticlepeer-review

40 Citations (Scopus)

Abstract

The effect of short-term creatine (Cr) supplementation upon content of skeletal muscle-derived-reactive oxygen species (ROS) was investigated. Wistar rats were supplemented with Cr (5 g/kg BW) or vehicle, by gavage, for 6 days. Soleus and extensor digitorum longus (EDL) muscles were removed and incubated for evaluation of ROS content using Amplex-UltraRed reagent. The analysis of expression and activity of antioxidant enzymes (superoxide dismutase 1 and 2, catalase and glutathione peroxidase) were performed. Direct scavenger action of Cr on superoxide radical and hydrogen peroxide was also investigated. Short-term Cr supplementation attenuated ROS content in both soleus and EDL muscles (by 41 and 33.7%, respectively). Cr supplementation did not change expression and activity of antioxidant enzymes. Basal TBARS content was not altered by Cr supplementation. In cell-free experiments, Cr showed a scavenger effect on superoxide radical in concentrations of 20 and 40 mM, but not on hydrogen peroxide. These results indicate that Cr supplementation decreases ROS content in skeletal muscle possibly due to a direct action of Cr molecule on superoxide radical.

Original languageEnglish
Pages (from-to)3905-3911
Number of pages7
JournalEuropean Journal of Applied Physiology
Volume112
Early online date14 Mar 2012
DOIs
Publication statusPublished - Nov 2012
Externally publishedYes

Bibliographical note

An Erratum to this article was published on 30 September 2012

Keywords

  • Creatine
  • Reactive Oxygen Species/metabolism
  • Superoxide Dismutase/genetics
  • nutritional supplements
  • dietary supplementation
  • Antioxidants

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