Sequence analysis of two porcine rotaviruses differing in growth in vitro and in pathogenicity: distinct VP4 sequences and conservation of NS53, VP6 and VP7 genes

B Burke, M A McCrae, U Desselberger

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

The VP4, VP7, NS53 and VP6 genes of two porcine rotavirus variants which differ in their in vitro growth properties and pathogenicity have been cloned and sequenced. The VP4 genes show only 67.2% nucleic acid and 70.6% amino acid identity. The VP4 gene of one variant (4S) is closely related to that of the bovine UK rotavirus strain, whereas the VP4 gene of the other variant (4F) is only distantly related to known VP4 genes and is likely to represent a new P serotype. In contrast the NS53 (VP5), VP6 and VP7 genes of the 4F and 4S variants show greater than 99% nucleotide and amino acid identity, indicating that the two viruses are genetically related by a reassortment event. The implications for the role of VP4 in the determination of in vitro growth characteristics and pathogenicity are discussed.

Original languageEnglish
Pages (from-to)2205-2212
Number of pages8
JournalThe Journal of general virology
Volume75
Issue number9
DOIs
Publication statusPublished - Sep 1994
Externally publishedYes

Fingerprint

Rotavirus
Sequence Analysis
Virulence
Swine
Growth
Genes
Amino Acids
Nucleic Acids
In Vitro Techniques
Nucleotides
Viruses

Keywords

  • Amino Acid Sequence
  • Animals
  • Antigens, Viral
  • Capsid/chemistry
  • Capsid Proteins
  • Cloning, Molecular
  • Conserved Sequence
  • DNA Primers
  • Genes, Viral
  • Genetic Variation
  • Molecular Sequence Data
  • Phylogeny
  • Polymerase Chain Reaction
  • RNA-Binding Proteins/chemistry
  • Rotavirus/genetics
  • Swine
  • Viral Nonstructural Proteins/chemistry

Cite this

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title = "Sequence analysis of two porcine rotaviruses differing in growth in vitro and in pathogenicity: distinct VP4 sequences and conservation of NS53, VP6 and VP7 genes",
abstract = "The VP4, VP7, NS53 and VP6 genes of two porcine rotavirus variants which differ in their in vitro growth properties and pathogenicity have been cloned and sequenced. The VP4 genes show only 67.2{\%} nucleic acid and 70.6{\%} amino acid identity. The VP4 gene of one variant (4S) is closely related to that of the bovine UK rotavirus strain, whereas the VP4 gene of the other variant (4F) is only distantly related to known VP4 genes and is likely to represent a new P serotype. In contrast the NS53 (VP5), VP6 and VP7 genes of the 4F and 4S variants show greater than 99{\%} nucleotide and amino acid identity, indicating that the two viruses are genetically related by a reassortment event. The implications for the role of VP4 in the determination of in vitro growth characteristics and pathogenicity are discussed.",
keywords = "Amino Acid Sequence, Animals, Antigens, Viral, Capsid/chemistry, Capsid Proteins, Cloning, Molecular, Conserved Sequence, DNA Primers, Genes, Viral, Genetic Variation, Molecular Sequence Data, Phylogeny, Polymerase Chain Reaction, RNA-Binding Proteins/chemistry, Rotavirus/genetics, Swine, Viral Nonstructural Proteins/chemistry",
author = "B Burke and McCrae, {M A} and U Desselberger",
year = "1994",
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doi = "10.1099/0022-1317-75-9-2205",
language = "English",
volume = "75",
pages = "2205--2212",
journal = "Journal of General Virology",
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T1 - Sequence analysis of two porcine rotaviruses differing in growth in vitro and in pathogenicity

T2 - distinct VP4 sequences and conservation of NS53, VP6 and VP7 genes

AU - Burke, B

AU - McCrae, M A

AU - Desselberger, U

PY - 1994/9

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N2 - The VP4, VP7, NS53 and VP6 genes of two porcine rotavirus variants which differ in their in vitro growth properties and pathogenicity have been cloned and sequenced. The VP4 genes show only 67.2% nucleic acid and 70.6% amino acid identity. The VP4 gene of one variant (4S) is closely related to that of the bovine UK rotavirus strain, whereas the VP4 gene of the other variant (4F) is only distantly related to known VP4 genes and is likely to represent a new P serotype. In contrast the NS53 (VP5), VP6 and VP7 genes of the 4F and 4S variants show greater than 99% nucleotide and amino acid identity, indicating that the two viruses are genetically related by a reassortment event. The implications for the role of VP4 in the determination of in vitro growth characteristics and pathogenicity are discussed.

AB - The VP4, VP7, NS53 and VP6 genes of two porcine rotavirus variants which differ in their in vitro growth properties and pathogenicity have been cloned and sequenced. The VP4 genes show only 67.2% nucleic acid and 70.6% amino acid identity. The VP4 gene of one variant (4S) is closely related to that of the bovine UK rotavirus strain, whereas the VP4 gene of the other variant (4F) is only distantly related to known VP4 genes and is likely to represent a new P serotype. In contrast the NS53 (VP5), VP6 and VP7 genes of the 4F and 4S variants show greater than 99% nucleotide and amino acid identity, indicating that the two viruses are genetically related by a reassortment event. The implications for the role of VP4 in the determination of in vitro growth characteristics and pathogenicity are discussed.

KW - Amino Acid Sequence

KW - Animals

KW - Antigens, Viral

KW - Capsid/chemistry

KW - Capsid Proteins

KW - Cloning, Molecular

KW - Conserved Sequence

KW - DNA Primers

KW - Genes, Viral

KW - Genetic Variation

KW - Molecular Sequence Data

KW - Phylogeny

KW - Polymerase Chain Reaction

KW - RNA-Binding Proteins/chemistry

KW - Rotavirus/genetics

KW - Swine

KW - Viral Nonstructural Proteins/chemistry

U2 - 10.1099/0022-1317-75-9-2205

DO - 10.1099/0022-1317-75-9-2205

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EP - 2212

JO - Journal of General Virology

JF - Journal of General Virology

SN - 0022-1317

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ER -