Selective effects of Lactobacillus casei Shirota on T cell activation, natural killer cell activity and cytokine production

H. Dong, I. Rowland, K.M. Tuohy, L.V. Thomas, P. Yaqoob

Research output: Contribution to journalArticle

56 Citations (Scopus)

Abstract

Summary
Modulation of host immunity is an important potential mechanism by which probiotics confer health benefits. This study was designed to investigate the effects of a probiotic strain, Lactobacillus casei Shirota (LcS), on immune function using human peripheral blood mononuclear cells (PBMC) in vitro. In addition, the role of monocytes in LcS‐induced immunity was also explored. LcS promoted natural killer (NK) cell activity and preferentially induced expression of CD69 and CD25 on CD8+ and CD56+ subsets in the absence of any other stimulus. LcS also induced production of interleukin (IL)‐1β, IL‐6, tumour necrosis factor (TNF)‐α, IL‐12 and IL‐10 in the absence of lipopolysaccharide (LPS). In the presence of LPS, LcS enhanced IL‐1β production but inhibited LPS‐induced IL‐10 and IL‐6 production, and had no further effect on TNF‐α and IL‐12 production. Monocyte depletion reduced significantly the impact of LcS on lymphocyte activation, cytokine production and natural killer (NK) cell activity. In conclusion, LcS activated cytotoxic lymphocytes preferentially in both the innate and specific immune systems, which suggests that LcS could potentiate the destruction of infected cells in the body. LcS also induced both proinflammatory and anti‐inflammatory cytokine production in the absence of LPS, but in some cases inhibited LPS‐induced cytokine production. Monocytes play an important role in LcS‐induced immunological responses.
Original languageEnglish
Pages (from-to)378-388
Number of pages11
JournalClinical and Experimental Immunology
Volume161
Issue number2
Early online date7 May 2010
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Keywords

  • cytokines
  • immunomodulation
  • L. casei Shirota
  • NK cell activity
  • T lymphocytes

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