Scalable 3D printed molds for human tissue engineered skeletal muscle

Andrew J. Capel; Rowan P. Rimington; Jacob W. Fleming; Darren J. Player; Luke A. Baker; Mark C. Turner; Julia M. Jones; Neil R.W. Martin; Richard A. Ferguson; Vivek C. Mudera; Mark P. Lewis

doi:10.3389/fbioe.2019.00020

Scalable 3D printed molds for human tissue engineered skeletal muscle

Andrew J. Capel, Rowan P. Rimington, Jacob W. Fleming, Darren J. Player, Luke A. Baker, Mark C. Turner, Julia M. Jones, Neil R.W. Martin, Richard A. Ferguson, Vivek C. Mudera, Mark P. Lewis

Research output: Contribution to journal › Article › peer-review

50 Citations (Scopus)

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Abstract

Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25-500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.

Original language	English
Article number	20
Number of pages	13
Journal	Frontiers in Bioengineering and Biotechnology
Volume	7
Issue number	Feb
DOIs	https://doi.org/10.3389/fbioe.2019.00020
Publication status	Published - 14 Feb 2019
Externally published	Yes

Bibliographical note

Copyright © 2019 Capel, Rimington, Fleming, Player, Baker, Turner, Jones, Martin, Ferguson, Mudera and Lewis. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

Keywords

3D printing
Bioengineering
Primary skeletal muscle
Skeletal muscle physiology
Tissue engineering

ASJC Scopus subject areas

Biotechnology
Bioengineering
Histology
Biomedical Engineering

Access to Document

10.3389/fbioe.2019.00020Licence: CC BY

PublishedFinal published version, 9.39 MBLicence: CC BY

Cite this

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title = "Scalable 3D printed molds for human tissue engineered skeletal muscle",

abstract = "Tissue engineered skeletal muscle allows investigation of the cellular and molecular mechanisms that regulate skeletal muscle pathology. The fabricated model must resemble characteristics of in vivo tissue and incorporate cost-effective and high content primary human tissue. Current models are limited by low throughput due to the complexities associated with recruiting tissue donors, donor specific variations, as well as cellular senescence associated with passaging. This research presents a method using fused deposition modeling (FDM) and laser sintering (LS) 3D printing to generate reproducible and scalable tissue engineered primary human muscle, possessing aligned mature myotubes reminiscent of in vivo tissue. Many existing models are bespoke causing variability when translated between laboratories. To this end, a scalable model has been developed (25-500 μL construct volumes) allowing fabrication of mature primary human skeletal muscle. This research provides a strategy to overcome limited biopsy cell numbers, enabling high throughput screening of functional human tissue.",

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Scalable 3D printed molds for human tissue engineered skeletal muscle

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

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