Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

Jamie K. Pugh; Steve H. Faulkner; Mark C. Turner; Myra A. Nimmo

doi:10.1007/s00421-017-3721-y

Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

Jamie K. Pugh, Steve H. Faulkner, Mark C. Turner, Myra A. Nimmo

Research output: Contribution to journal › Article › peer-review

17 Citations (Scopus)

35 Downloads (Pure)

Abstract

Purpose: Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Methods: Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HR_max on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7⁺ cells) and active (MyoD⁺ cells) satellite cell number using immunofluorescence microscopy. Results: Type-I-specific Pax7⁺ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD⁺ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7⁺ and MyoD⁺ cell number remained unchanged after both exercise trials (all P ≥ 0.13). Conclusion: Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.

Original language	English
Pages (from-to)	225-238
Number of pages	14
Journal	European Journal of Applied Physiology
Volume	118
Issue number	2
Early online date	25 Oct 2017
DOIs	https://doi.org/10.1007/s00421-017-3721-y
Publication status	Published - 1 Feb 2018
Externally published	Yes

Bibliographical note

This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the
source, provide a link to the Creative Commons license and indicate if changes were made.

Keywords

Acute responses
Concurrent exercise
High-intensity interval training
Human skeletal muscle
Interference
mRNA expression
Obesity
Resistance exercise
Satellite cell

ASJC Scopus subject areas

Orthopedics and Sports Medicine
Public Health, Environmental and Occupational Health
Physiology (medical)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.1007/s00421-017-3721-yLicence: CC BY

PublishedFinal published version, 4.5 MBLicence: CC BY

Cite this

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title = "Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals",

abstract = "Purpose: Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Methods: Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HRmax on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7+ cells) and active (MyoD+ cells) satellite cell number using immunofluorescence microscopy. Results: Type-I-specific Pax7+ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD+ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7+ and MyoD+ cell number remained unchanged after both exercise trials (all P ≥ 0.13). Conclusion: Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.",

keywords = "Acute responses, Concurrent exercise, High-intensity interval training, Human skeletal muscle, Interference, mRNA expression, Obesity, Resistance exercise, Satellite cell",

author = "Pugh, {Jamie K.} and Faulkner, {Steve H.} and Turner, {Mark C.} and Nimmo, {Myra A.}",

note = "This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made. ",

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AU - Pugh, Jamie K.

AU - Faulkner, Steve H.

AU - Turner, Mark C.

AU - Nimmo, Myra A.

N1 - This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits use, duplication, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license and indicate if changes were made.

PY - 2018/2/1

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N2 - Purpose: Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Methods: Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HRmax on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7+ cells) and active (MyoD+ cells) satellite cell number using immunofluorescence microscopy. Results: Type-I-specific Pax7+ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD+ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7+ and MyoD+ cell number remained unchanged after both exercise trials (all P ≥ 0.13). Conclusion: Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.

AB - Purpose: Sarcopenia can begin from the 4–5th decade of life and is exacerbated by obesity and inactivity. A combination of resistance exercise (RE) and endurance exercise is recommended to combat rising obesity and inactivity levels. However, work continues to elucidate whether interference in adaptive outcomes occur when RE and endurance exercise are performed concurrently. This study examined whether a single bout of concurrent RE and high-intensity interval training (HIIT) alters the satellite cell response following exercise compared to RE alone. Methods: Eight sedentary, overweight/obese, middle-aged individuals performed RE only (8 × 8 leg extensions at 70% 1RM), or RE + HIIT (10 × 1 min at 90% HRmax on a cycle ergometer). Muscle biopsies were collected from the vastus lateralis before and 96 h after the RE component to determine muscle fiber type-specific total (Pax7+ cells) and active (MyoD+ cells) satellite cell number using immunofluorescence microscopy. Results: Type-I-specific Pax7+ (P = 0.001) cell number increased after both exercise trials. Type-I-specific MyoD+ (P = 0.001) cell number increased after RE only. However, an elevated baseline value in RE + HIIT compared to RE (P = 0.046) was observed, with no differences between exercise trials at 96 h (P = 0.21). Type-II-specific Pax7+ and MyoD+ cell number remained unchanged after both exercise trials (all P ≥ 0.13). Conclusion: Combining a HIIT session after a single bout of RE does not interfere with the increase in type-I-specific total, and possibly active, satellite cell number, compared to RE only. Concurrent RE + HIIT may offer a time-efficient way to maximise the physiological benefits from a single bout of exercise in sedentary, overweight/obese, middle-aged individuals.

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KW - Human skeletal muscle

KW - Interference

KW - mRNA expression

KW - Obesity

KW - Resistance exercise

KW - Satellite cell

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Satellite cell response to concurrent resistance exercise and high-intensity interval training in sedentary, overweight/obese, middle-aged individuals

Abstract

Bibliographical note

Keywords

ASJC Scopus subject areas

UN SDGs

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