Reconstitution of solubilised brain D2 dopamine receptors into phospholipid vesicles

M. Wheatley, P. G. Strange

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

D2 dopamine receptors have been solubilised from bovine caudate nucleus using cholate/sodium chloride in the presence of soyabean phospholipid. Reconstitution of the receptors into soyabean phospholipid vesicles has been achieved by dialysis to remove detergent and salt. The receptors are truly reconstituted as judged by sedimentation, electron microscopy, heat stability and analysis on sucrose density gradients. The ligand-binding properties of the reconstituted receptors resemble those of the solubilised preparation.

Original languageEnglish
Pages (from-to)389-392
Number of pages4
JournalFEBS Letters
Volume166
Issue number2
DOIs
Publication statusPublished - 30 Jan 1984
Externally publishedYes

Bibliographical note

Free access

Keywords

  • D dopamine receptor
  • Reconstitution [H]Spiperone binding
  • Solubilisation

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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