Abstract
D2 dopamine receptors have been solubilised from bovine caudate nucleus using cholate/sodium chloride in the presence of soyabean phospholipid. Reconstitution of the receptors into soyabean phospholipid vesicles has been achieved by dialysis to remove detergent and salt. The receptors are truly reconstituted as judged by sedimentation, electron microscopy, heat stability and analysis on sucrose density gradients. The ligand-binding properties of the reconstituted receptors resemble those of the solubilised preparation.
Original language | English |
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Pages (from-to) | 389-392 |
Number of pages | 4 |
Journal | FEBS Letters |
Volume | 166 |
Issue number | 2 |
DOIs | |
Publication status | Published - 30 Jan 1984 |
Externally published | Yes |
Bibliographical note
Free accessKeywords
- D dopamine receptor
- Reconstitution [H]Spiperone binding
- Solubilisation
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology