Reconstitution of solubilised brain D2 dopamine receptors into phospholipid vesicles

M. Wheatley; P. G. Strange

doi:10.1016/0014-5793(84)80119-2

Reconstitution of solubilised brain D₂ dopamine receptors into phospholipid vesicles

M. Wheatley, P. G. Strange

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

D₂ dopamine receptors have been solubilised from bovine caudate nucleus using cholate/sodium chloride in the presence of soyabean phospholipid. Reconstitution of the receptors into soyabean phospholipid vesicles has been achieved by dialysis to remove detergent and salt. The receptors are truly reconstituted as judged by sedimentation, electron microscopy, heat stability and analysis on sucrose density gradients. The ligand-binding properties of the reconstituted receptors resemble those of the solubilised preparation.

Original language	English
Pages (from-to)	389-392
Number of pages	4
Journal	FEBS Letters
Volume	166
Issue number	2
DOIs	https://doi.org/10.1016/0014-5793(84)80119-2
Publication status	Published - 30 Jan 1984
Externally published	Yes

Bibliographical note

Free access

Keywords

D dopamine receptor
Reconstitution [H]Spiperone binding
Solubilisation

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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10.1016/0014-5793(84)80119-2

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