Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability

S Shen, B Burke, U Desselberger

    Research output: Contribution to journalArticlepeer-review

    57 Citations (Scopus)

    Abstract

    A group A rotavirus isolated from a lamb with diarrhea in Qinhai province, China, was serially passaged in fetal calf kidney cells. In passage 96, rearrangements of RNA segments 5 and 6 of the viral genome were found. Here we report the nucleotide and predicted amino acid sequences of normal and rearranged RNA 6, coding for the major inner capsid protein VP6. In comparison with the normal gene (N6), the rearranged RNA 6 (R6) contained the normal open reading frame followed by a 473-nucleotide (nt) duplication of the gene beginning 23 nt after the termination codon. The duplicated region starts at nt 768 and runs through to the 3' end of the gene. In accordance with the nucleotide sequence of the rearranged RNA 6, a normal-length VP6 product was found in cells infected with the mutant. However, a single-amino-acid change from proline to glutamine at position 309 slightly affected the electrophoretic mobility of the VP6 monomer of the R6 mutant and reduced the stability of VP6 trimers on gels and at low pH values compared with the normal gene product. The degree of relatedness of VP6 of the Chinese lamb rotavirus Lp14 to those of other group A rotaviruses was determined.

    Original languageEnglish
    Pages (from-to)1682-1688
    Number of pages7
    JournalJournal of Virology
    Volume68
    Issue number3
    Publication statusPublished - Mar 1994

    Keywords

    • Animals
    • Antigens, Viral
    • Base Sequence
    • Capsid/genetics
    • Capsid Proteins
    • China/epidemiology
    • Gene Rearrangement
    • Genes, Viral/genetics
    • Genome, Viral
    • Molecular Sequence Data
    • Point Mutation
    • Polymerase Chain Reaction
    • Protein Conformation
    • Protein Denaturation
    • RNA, Messenger/isolation & purification
    • RNA, Viral/genetics
    • RNA-Directed DNA Polymerase
    • Rotavirus/genetics
    • Rotavirus Infections/epidemiology
    • Sequence Analysis, DNA
    • Sheep
    • Transcription, Genetic
    • Viral Proteins/analysis

    Fingerprint

    Dive into the research topics of 'Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability'. Together they form a unique fingerprint.

    Cite this