Abstract
A group A rotavirus isolated from a lamb with diarrhea in Qinhai province, China, was serially passaged in fetal calf kidney cells. In passage 96, rearrangements of RNA segments 5 and 6 of the viral genome were found. Here we report the nucleotide and predicted amino acid sequences of normal and rearranged RNA 6, coding for the major inner capsid protein VP6. In comparison with the normal gene (N6), the rearranged RNA 6 (R6) contained the normal open reading frame followed by a 473-nucleotide (nt) duplication of the gene beginning 23 nt after the termination codon. The duplicated region starts at nt 768 and runs through to the 3' end of the gene. In accordance with the nucleotide sequence of the rearranged RNA 6, a normal-length VP6 product was found in cells infected with the mutant. However, a single-amino-acid change from proline to glutamine at position 309 slightly affected the electrophoretic mobility of the VP6 monomer of the R6 mutant and reduced the stability of VP6 trimers on gels and at low pH values compared with the normal gene product. The degree of relatedness of VP6 of the Chinese lamb rotavirus Lp14 to those of other group A rotaviruses was determined.
| Original language | English |
|---|---|
| Pages (from-to) | 1682-1688 |
| Number of pages | 7 |
| Journal | Journal of Virology |
| Volume | 68 |
| Issue number | 3 |
| Publication status | Published - Mar 1994 |
Fingerprint
Keywords
- Animals
- Antigens, Viral
- Base Sequence
- Capsid/genetics
- Capsid Proteins
- China/epidemiology
- Gene Rearrangement
- Genes, Viral/genetics
- Genome, Viral
- Molecular Sequence Data
- Point Mutation
- Polymerase Chain Reaction
- Protein Conformation
- Protein Denaturation
- RNA, Messenger/isolation & purification
- RNA, Viral/genetics
- RNA-Directed DNA Polymerase
- Rotavirus/genetics
- Rotavirus Infections/epidemiology
- Sequence Analysis, DNA
- Sheep
- Transcription, Genetic
- Viral Proteins/analysis
Cite this
Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability. / Shen, S; Burke, B; Desselberger, U.
In: Journal of Virology, Vol. 68, No. 3, 03.1994, p. 1682-1688.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Rearrangement of the VP6 gene of a group A rotavirus in combination with a point mutation affecting trimer stability
AU - Shen, S
AU - Burke, B
AU - Desselberger, U
PY - 1994/3
Y1 - 1994/3
N2 - A group A rotavirus isolated from a lamb with diarrhea in Qinhai province, China, was serially passaged in fetal calf kidney cells. In passage 96, rearrangements of RNA segments 5 and 6 of the viral genome were found. Here we report the nucleotide and predicted amino acid sequences of normal and rearranged RNA 6, coding for the major inner capsid protein VP6. In comparison with the normal gene (N6), the rearranged RNA 6 (R6) contained the normal open reading frame followed by a 473-nucleotide (nt) duplication of the gene beginning 23 nt after the termination codon. The duplicated region starts at nt 768 and runs through to the 3' end of the gene. In accordance with the nucleotide sequence of the rearranged RNA 6, a normal-length VP6 product was found in cells infected with the mutant. However, a single-amino-acid change from proline to glutamine at position 309 slightly affected the electrophoretic mobility of the VP6 monomer of the R6 mutant and reduced the stability of VP6 trimers on gels and at low pH values compared with the normal gene product. The degree of relatedness of VP6 of the Chinese lamb rotavirus Lp14 to those of other group A rotaviruses was determined.
AB - A group A rotavirus isolated from a lamb with diarrhea in Qinhai province, China, was serially passaged in fetal calf kidney cells. In passage 96, rearrangements of RNA segments 5 and 6 of the viral genome were found. Here we report the nucleotide and predicted amino acid sequences of normal and rearranged RNA 6, coding for the major inner capsid protein VP6. In comparison with the normal gene (N6), the rearranged RNA 6 (R6) contained the normal open reading frame followed by a 473-nucleotide (nt) duplication of the gene beginning 23 nt after the termination codon. The duplicated region starts at nt 768 and runs through to the 3' end of the gene. In accordance with the nucleotide sequence of the rearranged RNA 6, a normal-length VP6 product was found in cells infected with the mutant. However, a single-amino-acid change from proline to glutamine at position 309 slightly affected the electrophoretic mobility of the VP6 monomer of the R6 mutant and reduced the stability of VP6 trimers on gels and at low pH values compared with the normal gene product. The degree of relatedness of VP6 of the Chinese lamb rotavirus Lp14 to those of other group A rotaviruses was determined.
KW - Animals
KW - Antigens, Viral
KW - Base Sequence
KW - Capsid/genetics
KW - Capsid Proteins
KW - China/epidemiology
KW - Gene Rearrangement
KW - Genes, Viral/genetics
KW - Genome, Viral
KW - Molecular Sequence Data
KW - Point Mutation
KW - Polymerase Chain Reaction
KW - Protein Conformation
KW - Protein Denaturation
KW - RNA, Messenger/isolation & purification
KW - RNA, Viral/genetics
KW - RNA-Directed DNA Polymerase
KW - Rotavirus/genetics
KW - Rotavirus Infections/epidemiology
KW - Sequence Analysis, DNA
KW - Sheep
KW - Transcription, Genetic
KW - Viral Proteins/analysis
M3 - Article
VL - 68
SP - 1682
EP - 1688
JO - Virology
JF - Virology
SN - 0042-6822
IS - 3
ER -