Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering

Kornelia Jumel; Immo Fiebrig; Stephen E. Harding

doi:10.1016/0141-8130(95)01071-8

Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering

Kornelia Jumel, Immo Fiebrig, Stephen E. Harding

University of Nottingham

Research output: Contribution to journal › Article › peer-review

75 Citations (Scopus)

Abstract

The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand, a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.

Original language	English
Pages (from-to)	133-139
Number of pages	7
Journal	International Journal of Biological Macromolecules
Volume	18
Issue number	1-2
DOIs	https://doi.org/10.1016/0141-8130(95)01071-8
Publication status	Published - Feb 1996
Externally published	Yes

Keywords

Molar mass determination
Mucus glycoproteins
Size exclusion chromatography/multi angle laser light scattering

ASJC Scopus subject areas

Biochemistry
Molecular Biology

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10.1016/0141-8130(95)01071-8

Cite this

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abstract = "The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand, a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.",

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AU - Fiebrig, Immo

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AB - The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand, a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.

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KW - Mucus glycoproteins

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Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering

Abstract

Keywords

ASJC Scopus subject areas

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