Rapid size distribution and purity analysis of gastric mucus glycoproteins by size exclusion chromatography/multi angle laser light scattering

Kornelia Jumel, Immo Fiebrig, Stephen E. Harding

Research output: Contribution to journalArticle

72 Citations (Scopus)

Abstract

The weight average molar masses and molar mass distributions of two commercial pig gastric mucins and two fresh mucin preparations were determined by the technique of size exclusion chromatography coupled on-line to multi angle laser light scattering (SEC/MALLS). Both commercial samples exhibited much lower molar mass averages than the freshly prepared material and contained more impurities. On the other hand, a fresh mucin preparation examined after being stored frozen for 18 months revealed a slight increase in molar mass.

Original languageEnglish
Pages (from-to)133-139
Number of pages7
JournalInternational Journal of Biological Macromolecules
Volume18
Issue number1-2
DOIs
Publication statusPublished - Feb 1996
Externally publishedYes

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Keywords

  • Molar mass determination
  • Mucus glycoproteins
  • Size exclusion chromatography/multi angle laser light scattering

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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