Quantification of MRI and MRS characteristics changes in a rat model at different stage of cerebral ischemia

Yan Zhang; Dongmei Hao; Xiuhua Lv; Shaowu Li; Yunqing Tian; Dingchang Zheng; Yanjun Zeng

doi:10.1080/01616412.2016.1181345

Quantification of MRI and MRS characteristics changes in a rat model at different stage of cerebral ischemia

Yan Zhang, Dongmei Hao, Xiuhua Lv, Shaowu Li, Yunqing Tian, Dingchang Zheng, Yanjun Zeng

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Abstract

Background: A better understanding the mechanisms of cerebral ischemia is important both for diagnosis and treatment. Objective: The study aimed to quantify several characteristics of magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to indicate the brain tissue changes at different stage of cerebral ischemia in rats. Methods: In the present study, a rat model of cerebral ischemia was established by middle cerebral artery occlusion (MCAO) in the left hemisphere. MRI and MRS were performed on 15 Sprague Dawley rats 4 H, 24 H, and 1 W after MCAO. Apparent diffusion coefficient (ADC), relative ADC including FNR, PNR, PNF, and metabolite ratio NCC were proposed to reflect the changes of water diffusion and metabolism in brain tissue. Results: ADCs of focal zone and penumbra zone from 1 W group were significantly larger than those from 4H group, respectively (both p < 0.05). PNR and PNF of 24H and 1 W groups were significantly less than 4H group (all p < 0.01). NCCs of focal zone and penumbra zone were significantly less than the normal zone within 4H, 24H, and 1 W groups, respectively (both p < 0.01). While NCCs of penumbra zone from 24H and 1 W groups were significantly larger than 4H group (both p < 0.01). Conclusion: We conclude that combination of MRI and MRS characteristics can provide significant indicators for ischemic damage at different stage of cerebral ischemia in a rat model.

Original language	English
Pages (from-to)	640-646
Number of pages	7
Journal	Neurological Research
Volume	38
Issue number	7
Early online date	23 May 2016
DOIs	https://doi.org/10.1080/01616412.2016.1181345
Publication status	Published - 2016
Externally published	Yes

Bibliographical note

Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

Keywords

State intellectural property office
Magnetic resonance spectroscopy (MRS)
Cerebral ischemia
Middle cerebral artery occlusion (MCAO)
Rat

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10.1080/01616412.2016.1181345

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title = "Quantification of MRI and MRS characteristics changes in a rat model at different stage of cerebral ischemia",

abstract = "Background: A better understanding the mechanisms of cerebral ischemia is important both for diagnosis and treatment. Objective: The study aimed to quantify several characteristics of magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to indicate the brain tissue changes at different stage of cerebral ischemia in rats. Methods: In the present study, a rat model of cerebral ischemia was established by middle cerebral artery occlusion (MCAO) in the left hemisphere. MRI and MRS were performed on 15 Sprague Dawley rats 4 H, 24 H, and 1 W after MCAO. Apparent diffusion coefficient (ADC), relative ADC including FNR, PNR, PNF, and metabolite ratio NCC were proposed to reflect the changes of water diffusion and metabolism in brain tissue. Results: ADCs of focal zone and penumbra zone from 1 W group were significantly larger than those from 4H group, respectively (both p < 0.05). PNR and PNF of 24H and 1 W groups were significantly less than 4H group (all p < 0.01). NCCs of focal zone and penumbra zone were significantly less than the normal zone within 4H, 24H, and 1 W groups, respectively (both p < 0.01). While NCCs of penumbra zone from 24H and 1 W groups were significantly larger than 4H group (both p < 0.01). Conclusion: We conclude that combination of MRI and MRS characteristics can provide significant indicators for ischemic damage at different stage of cerebral ischemia in a rat model.",

keywords = "State intellectural property office, Magnetic resonance spectroscopy (MRS), Cerebral ischemia, Middle cerebral artery occlusion (MCAO), Rat",

author = "Yan Zhang and Dongmei Hao and Xiuhua Lv and Shaowu Li and Yunqing Tian and Dingchang Zheng and Yanjun Zeng",

note = "Copyright {\textcopyright} and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders. ",

year = "2016",

doi = "10.1080/01616412.2016.1181345",

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T1 - Quantification of MRI and MRS characteristics changes in a rat model at different stage of cerebral ischemia

AU - Zhang, Yan

AU - Hao, Dongmei

AU - Lv, Xiuhua

AU - Li, Shaowu

AU - Tian, Yunqing

AU - Zheng, Dingchang

AU - Zeng, Yanjun

N1 - Copyright © and Moral Rights are retained by the author(s) and/ or other copyright owners. A copy can be downloaded for personal non-commercial research or study, without prior permission or charge. This item cannot be reproduced or quoted extensively from without first obtaining permission in writing from the copyright holder(s). The content must not be changed in any way or sold commercially in any format or medium without the formal permission of the copyright holders.

PY - 2016

Y1 - 2016

N2 - Background: A better understanding the mechanisms of cerebral ischemia is important both for diagnosis and treatment. Objective: The study aimed to quantify several characteristics of magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to indicate the brain tissue changes at different stage of cerebral ischemia in rats. Methods: In the present study, a rat model of cerebral ischemia was established by middle cerebral artery occlusion (MCAO) in the left hemisphere. MRI and MRS were performed on 15 Sprague Dawley rats 4 H, 24 H, and 1 W after MCAO. Apparent diffusion coefficient (ADC), relative ADC including FNR, PNR, PNF, and metabolite ratio NCC were proposed to reflect the changes of water diffusion and metabolism in brain tissue. Results: ADCs of focal zone and penumbra zone from 1 W group were significantly larger than those from 4H group, respectively (both p < 0.05). PNR and PNF of 24H and 1 W groups were significantly less than 4H group (all p < 0.01). NCCs of focal zone and penumbra zone were significantly less than the normal zone within 4H, 24H, and 1 W groups, respectively (both p < 0.01). While NCCs of penumbra zone from 24H and 1 W groups were significantly larger than 4H group (both p < 0.01). Conclusion: We conclude that combination of MRI and MRS characteristics can provide significant indicators for ischemic damage at different stage of cerebral ischemia in a rat model.

AB - Background: A better understanding the mechanisms of cerebral ischemia is important both for diagnosis and treatment. Objective: The study aimed to quantify several characteristics of magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) to indicate the brain tissue changes at different stage of cerebral ischemia in rats. Methods: In the present study, a rat model of cerebral ischemia was established by middle cerebral artery occlusion (MCAO) in the left hemisphere. MRI and MRS were performed on 15 Sprague Dawley rats 4 H, 24 H, and 1 W after MCAO. Apparent diffusion coefficient (ADC), relative ADC including FNR, PNR, PNF, and metabolite ratio NCC were proposed to reflect the changes of water diffusion and metabolism in brain tissue. Results: ADCs of focal zone and penumbra zone from 1 W group were significantly larger than those from 4H group, respectively (both p < 0.05). PNR and PNF of 24H and 1 W groups were significantly less than 4H group (all p < 0.01). NCCs of focal zone and penumbra zone were significantly less than the normal zone within 4H, 24H, and 1 W groups, respectively (both p < 0.01). While NCCs of penumbra zone from 24H and 1 W groups were significantly larger than 4H group (both p < 0.01). Conclusion: We conclude that combination of MRI and MRS characteristics can provide significant indicators for ischemic damage at different stage of cerebral ischemia in a rat model.

KW - State intellectural property office

KW - Magnetic resonance spectroscopy (MRS)

KW - Cerebral ischemia

KW - Middle cerebral artery occlusion (MCAO)

KW - Rat

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DO - 10.1080/01616412.2016.1181345

M3 - Article

SN - 1743-1328

VL - 38

SP - 640

EP - 646

JO - Neurological Research

JF - Neurological Research

IS - 7

ER -

Quantification of MRI and MRS characteristics changes in a rat model at different stage of cerebral ischemia

Abstract

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