Protocol: precision engineering of plant gene loci by homologous recombination cloning in Escherichia coli.

LC Roden, B Göttgens, ES Mutasa-Göttgens

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Plant genome sequence data now provide opportunities to conduct molecular genetic studies at the level of the whole gene locus and above. Such studies will be greatly facilitated by adopting and developing further the new generation of genetic engineering tools, based on homologous recombination cloning in Escherichia coli, which are free from the constraints imposed by the availability of suitably positioned restriction sites. Here we describe the basis for homologous recombination cloning in E. coli, the available tools and resources, together with a protocol for long range cloning and manipulation of an Arabidopsis thaliana gene locus, to create constructs co-ordinately driven by locus-specific regulatory elements.
Original languageEnglish
Number of pages12
JournalPlant Methods
Volume1
Issue number6
DOIs
Publication statusPublished - 29 Sep 2005
Externally publishedYes

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