Abstract
| Original language | English |
|---|---|
| Pages (from-to) | 235-245 |
| Number of pages | 11 |
| Journal | European Journal of Pharmacology |
| Volume | 477 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 23 Sep 2003 |
Fingerprint
Keywords
- Adenosine A3 receptor
- Ischaemia
- Hypoxia
- Stunning
- Heart
- isolated
- working
- Papillary muscle
- Atrium
- Cardioprotection
Cite this
Protection from myocardial stunning by ischaemia and hypoxia with the adenosine A3 receptor agonist, IB-MECA. / Maddock, H.L.; Gardner, N.M.; Khandoudi, N.; Bril, A.; Broadley, K.J.
In: European Journal of Pharmacology, Vol. 477, No. 3, 23.09.2003, p. 235-245.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Protection from myocardial stunning by ischaemia and hypoxia with the adenosine A3 receptor agonist, IB-MECA
AU - Maddock, H.L.
AU - Gardner, N.M.
AU - Khandoudi, N.
AU - Bril, A.
AU - Broadley, K.J.
PY - 2003/9/23
Y1 - 2003/9/23
N2 - Guinea pig isolated working hearts were exposed to 30-min ischaemia by reducing coronary flow to 10%, followed by reperfusion. Aortic output fell to 4.5±4.5% of the pre-ischaemic value at reperfusion, recovering to 48.2±14.6% at 20-min post-reperfusion; the index of myocardial stunning. IB-MECA (N6-(3-iodobenzyl)adenosine-5′-N-methyluronamide, 3×10−7 M), infused from 10 min into ischaemia, did not affect recovery of aortic output 20 min after reperfusion (41.9±1.9%). IB-MECA infused at reperfusion, however, significantly protected against stunning, aortic output recovering to 79.6±3.9% at 20-min post-reperfusion. Hypoxic gassing (5% CO2 in nitrogen, 30 min) of guinea pig isolated paced left atria and papillary muscles reduced the developed tension, recovering to 75% 5 min after re-oxygenation. This myocardial stunning was unaffected by IB-MECA (3×10−7 M) added 10 min into hypoxia. IB-MECA added at reoxygenation significantly improved recovery, which was prevented by the adenosine A3 receptor antagonist, 1-propyl-3-(3-iodo-4-aminobenzyl)-8-(4-oxyacetate)phenylxanthine (I-ABOPX, 1×10−5 M). Thus, stimulation of adenosine A3 receptors at reperfusion/reoxygenation in guinea pig cardiac preparations protects against myocardial stunning.
AB - Guinea pig isolated working hearts were exposed to 30-min ischaemia by reducing coronary flow to 10%, followed by reperfusion. Aortic output fell to 4.5±4.5% of the pre-ischaemic value at reperfusion, recovering to 48.2±14.6% at 20-min post-reperfusion; the index of myocardial stunning. IB-MECA (N6-(3-iodobenzyl)adenosine-5′-N-methyluronamide, 3×10−7 M), infused from 10 min into ischaemia, did not affect recovery of aortic output 20 min after reperfusion (41.9±1.9%). IB-MECA infused at reperfusion, however, significantly protected against stunning, aortic output recovering to 79.6±3.9% at 20-min post-reperfusion. Hypoxic gassing (5% CO2 in nitrogen, 30 min) of guinea pig isolated paced left atria and papillary muscles reduced the developed tension, recovering to 75% 5 min after re-oxygenation. This myocardial stunning was unaffected by IB-MECA (3×10−7 M) added 10 min into hypoxia. IB-MECA added at reoxygenation significantly improved recovery, which was prevented by the adenosine A3 receptor antagonist, 1-propyl-3-(3-iodo-4-aminobenzyl)-8-(4-oxyacetate)phenylxanthine (I-ABOPX, 1×10−5 M). Thus, stimulation of adenosine A3 receptors at reperfusion/reoxygenation in guinea pig cardiac preparations protects against myocardial stunning.
KW - Adenosine A3 receptor
KW - Ischaemia
KW - Hypoxia
KW - Stunning
KW - Heart
KW - isolated
KW - working
KW - Papillary muscle
KW - Atrium
KW - Cardioprotection
UR - http://www.scopus.com/inward/record.url?eid=2-s2.0-1642279640&partnerID=MN8TOARS
U2 - 10.1016/j.ejphar.2003.08.024
DO - 10.1016/j.ejphar.2003.08.024
M3 - Article
VL - 477
SP - 235
EP - 245
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 3
ER -