Production of human allotype-specific anti-CD45 monoclonal antibodies

Gillian S. Dean, Richard Aspinall

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

The aim of this work was to raise allotype specific monoclonal antibodies to human CD45, with the long-term objective of producing a reagent which could be used to prolong graft survival in renal transplantation through removal of passenger leukocytes from the graft. At present there are no anti-CD45 monoclonal antibodies able to distinguish between host and donor leukocytes. An in vitro immunisation technique has been developed through which donated human-leukocytes are sensitised to CD45 prior to fusion with a myeloma cell line. IgM was produced by all the anti-CD45-positive clones. Flow cytometric analysis using these antibodies showed their ability to differentiate between blood from individual donors, indicating the existence of allotypic forms of human CD45, in conformity with the findings in rats and pigs. Therefore, a reagent which could be used in renal transplantation is a technical possibility.

Original languageEnglish
Pages (from-to)333-337
Number of pages5
JournalImmunology Letters
Volume68
Issue number2-3
DOIs
Publication statusPublished - 1 Jun 1999
Externally publishedYes

Fingerprint

Leukocytes
Monoclonal Antibodies
Kidney Transplantation
Graft Survival
Blood Donors
Immunoglobulin M
Immunization
Swine
Clone Cells
Tissue Donors
Transplants
Cell Line
Antibodies
In Vitro Techniques

Keywords

  • Allotype-specific
  • CD45
  • Human
  • Monoclonal antibody

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Production of human allotype-specific anti-CD45 monoclonal antibodies. / Dean, Gillian S.; Aspinall, Richard.

In: Immunology Letters, Vol. 68, No. 2-3, 01.06.1999, p. 333-337.

Research output: Contribution to journalArticle

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