PLIT: An alignment-free computational tool for identification of long non-coding RNAs in plant transcriptomic datasets

Sumukh Deshpande, James Shuttleworth, Jianhua Yang, Sandy Taramonli, Matthew England

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Long non-coding RNAs (lncRNAs) are a class of non-coding RNAs which play a significant role in several biological processes. RNA-seq based transcriptome sequencing has been extensively used for identification of lncRNAs. However, accurate identification of lncRNAs in RNA-seq datasets is crucial for exploring their characteristic functions in the genome as most coding potential computation (CPC) tools fail to accurately identify them in transcriptomic data. Well-known CPC tools such as CPC2, lncScore, CPAT are primarily designed for prediction of lncRNAs based on the GENCODE, NONCODE and CANTATAdb databases. The prediction accuracy of these tools often drops when tested on transcriptomic datasets. This leads to higher false positive results and inaccuracy in the function annotation process. In this study, we present a novel tool, PLIT, for the identification of lncRNAs in plants RNA-seq datasets. PLIT implements a feature selection method based on L1 regularization and iterative Random Forests (iRF) classification for selection of optimal features. Based on sequence and codon-bias features, it classifies the RNA-seq derived FASTA sequences into coding or long non-coding transcripts. Using L1 regularization, 31 optimal features were obtained based on lncRNA and protein-coding transcripts from 8 plant species. The performance of the tool was evaluated on 7 plant RNA-seq datasets using 10-fold cross-validation. The analysis exhibited superior accuracy when evaluated against currently available state-of-the-art CPC tools.
Original languageEnglish
Pages (from-to)169-181
Number of pages13
JournalComputers in Biology and Medicine
Volume105
Early online date4 Jan 2019
DOIs
Publication statusPublished - Feb 2019

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RNA
Feature extraction
Genes
Proteins

Keywords

  • CANTATAdb
  • Ensembl plants
  • Iterative random forests
  • LASSO
  • lncRNA
  • Random forests
  • RNA-seq

ASJC Scopus subject areas

  • Computer Science Applications
  • Health Informatics

Cite this

PLIT: An alignment-free computational tool for identification of long non-coding RNAs in plant transcriptomic datasets. / Deshpande, Sumukh; Shuttleworth, James; Yang, Jianhua; Taramonli, Sandy; England, Matthew.

In: Computers in Biology and Medicine, Vol. 105, 02.2019, p. 169-181.

Research output: Contribution to journalArticle

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