Phylogenetics of Theileria species in small ruminants

Olivier A.E. Sparagano, Eva Spitalska, Mehdi Namavari, Alessandra Torina, Vincenza Cannella, Santo Caracappa

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

10 Citations (Scopus)

Abstract

Our study is based on the collection of blood and ticks from sheep in Iran and Italy. Polymerase chain reaction (PCR) testing was performed to target the 18S rRNA gene and RLB was performed using previously published probes. In Italy and Iran 78.7% and 76.0% of the sheep were PCR positive, which after sequencing and RLB showed that they were Theileria ovis and Theileria lestoquardi, respectively. Phylogenetic analysis was performed using the Clustal W multiple sequence alignment program and our sequences were compared with more than 50 others already published in the EMBL database. Our T. lestoquardi sequences linked with other T. lestoquardi sequences from Iran, Tanzania, and Sudan and Theileria annulata showed the importance of having species-specific probes between these two species. However, distinctive clades were found between T. lestoquardi ticks and those found in sheep blood. Italian T. ovis seemed to be closer to Theileria spp. from Namibia and Iran than with other T. ovis from Spain, Turkey, Tanzania, and Sudan adding some information to the controversy about this species. However, some confusion was found on the existing database where the location of pathogens, years, and species names was inaccurate and when available sequences were not always appropriately used. This article will discuss our results and some comparisons with other phylogenetic approaches.

Original languageEnglish
Title of host publicationImpact of Emerging Zoonotic Diseases on Animal Health
Subtitle of host publication8th Biennial Conference of the Society for Tropical Veterinary Medicine
PublisherWiley
Pages505-508
Number of pages4
Volume1081
Edition1
ISBN (Print)1573316377, 9781573316378
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1081
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Theileria
Polymerase chain reaction
Ruminants
Sheep
Blood
Iran
Pathogens
Sudan
Tanzania
Genes
Ticks
Italy
Testing
Theileria annulata
Namibia
Databases
Polymerase Chain Reaction
Sequence Alignment
Turkey
rRNA Genes

Keywords

  • Diagnostic
  • Sheep
  • Theileria lestoquardi
  • Theileria ovis
  • Theileriosis
  • Tick

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Sparagano, O. A. E., Spitalska, E., Namavari, M., Torina, A., Cannella, V., & Caracappa, S. (2006). Phylogenetics of Theileria species in small ruminants. In Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine (1 ed., Vol. 1081, pp. 505-508). (Annals of the New York Academy of Sciences; Vol. 1081). Wiley. https://doi.org/10.1196/annals.1373.075

Phylogenetics of Theileria species in small ruminants. / Sparagano, Olivier A.E.; Spitalska, Eva; Namavari, Mehdi; Torina, Alessandra; Cannella, Vincenza; Caracappa, Santo.

Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. Vol. 1081 1. ed. Wiley, 2006. p. 505-508 (Annals of the New York Academy of Sciences; Vol. 1081).

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

Sparagano, OAE, Spitalska, E, Namavari, M, Torina, A, Cannella, V & Caracappa, S 2006, Phylogenetics of Theileria species in small ruminants. in Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. 1 edn, vol. 1081, Annals of the New York Academy of Sciences, vol. 1081, Wiley, pp. 505-508. https://doi.org/10.1196/annals.1373.075
Sparagano OAE, Spitalska E, Namavari M, Torina A, Cannella V, Caracappa S. Phylogenetics of Theileria species in small ruminants. In Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. 1 ed. Vol. 1081. Wiley. 2006. p. 505-508. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1373.075
Sparagano, Olivier A.E. ; Spitalska, Eva ; Namavari, Mehdi ; Torina, Alessandra ; Cannella, Vincenza ; Caracappa, Santo. / Phylogenetics of Theileria species in small ruminants. Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. Vol. 1081 1. ed. Wiley, 2006. pp. 505-508 (Annals of the New York Academy of Sciences).
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