Pervasive Differential Splicing in Marek’s Disease Virus can Discriminate CVI-988 Vaccine Strain from RB-1B Very Virulent Strain in Chicken Embryonic Fibroblasts

Yashar Sadigh; Abdessamad Tahiri-Alaoui; Stephen Spatz; Venugopal Nair; Paolo Ribeca

doi:10.3390/v12030329

Pervasive Differential Splicing in Marek’s Disease Virus can Discriminate CVI-988 Vaccine Strain from RB-1B Very Virulent Strain in Chicken Embryonic Fibroblasts

Yashar Sadigh, Abdessamad Tahiri-Alaoui, Stephen Spatz, Venugopal Nair, Paolo Ribeca

Research output: Contribution to journal › Article › peer-review

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Abstract

Marek’s disease is a major scourge challenging poultry health worldwide. It is caused by the highly contagious Marek’s disease virus (MDV), an alphaherpesvirus. Here, we showed that, similar to other members of its Herpesviridae family, MDV also presents a complex landscape of splicing events, most of which are uncharacterised and/or not annotated. Quite strikingly, and although the biological relevance of this fact is unknown, we found that a number of viral splicing isoforms are strain-specific, despite the close sequence similarity of the strains considered: very virulent RB-1B and vaccine CVI-988. We validated our findings by devising an assay that discriminated infections caused by the two strains in chicken embryonic fibroblasts on the basis of the presence of some RNA species. To our knowledge, this study is the first to accomplish such a result, emphasizing how relevant a comprehensive picture of the viral transcriptome is to fully understand viral pathogenesis.

Original language	English
Article number	329
Number of pages	20
Journal	Viruses
Volume	12
Issue number	3
DOIs	https://doi.org/10.3390/v12030329
Publication status	Published - 18 Mar 2020
Externally published	Yes

Bibliographical note

Funder

The Pirbright Institute receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom (projects BB/E/I/00007035, BB/E/I/00007036,
BB/E/I/00007037, BB/E/I/00007038, and BBS/E/I/00007039). We also acknowledge support from BBSRC grants
BBS/E/I/00001942 and BB/K011057/1. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This work was funded in part by USDA-ARS CRIS project number 6040-32000-074-00D

Funding

The Pirbright Institute receives grant-aided support from the Biotechnology and Biological Sciences Research Council of the United Kingdom (projects BB/E/I/00007035, BB/E/I/00007036, BB/E/I/00007037, BB/E/I/00007038, and BBS/E/I/00007039). We also acknowledge support from BBSRC grants BBS/E/I/00001942 and BB/K011057/1. Mention of trade names or commercial products in this publication is solely for the purpose of providing specific information and does not imply recommendation or endorsement by the U.S. Department of Agriculture. This work was funded in part by USDA-ARS CRIS project number 6040-32000-074-00D

Funders	Funder number
UK Research and Innovation
Biotechnology and Biological Sciences Research Council	BBS/E/I/00007039, BBS/E/I/00001942, BB/E/I/00007038, BBS/E/I/0000732, BB/E/I/00007037, BB/E/I/00007036, BBS/E/I/0000734, BB/E/I/00007035, BB/K011057/1
US Department of Agriculture	6040-32000-074-00D

Keywords

MDV strain CVI-988
MDV strain RB-1B
Marek's disease virus
RNA splicing isoforms
Transcriptomics

ASJC Scopus subject areas

Infectious Diseases
Virology

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

Access to Document

10.3390/v12030329Licence: CC BY

PublishedFinal published version, 1.37 MBLicence: CC BY

Cite this

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title = "Pervasive Differential Splicing in Marek{\textquoteright}s Disease Virus can Discriminate CVI-988 Vaccine Strain from RB-1B Very Virulent Strain in Chicken Embryonic Fibroblasts",

abstract = "Marek{\textquoteright}s disease is a major scourge challenging poultry health worldwide. It is caused by the highly contagious Marek{\textquoteright}s disease virus (MDV), an alphaherpesvirus. Here, we showed that, similar to other members of its Herpesviridae family, MDV also presents a complex landscape of splicing events, most of which are uncharacterised and/or not annotated. Quite strikingly, and although the biological relevance of this fact is unknown, we found that a number of viral splicing isoforms are strain-specific, despite the close sequence similarity of the strains considered: very virulent RB-1B and vaccine CVI-988. We validated our findings by devising an assay that discriminated infections caused by the two strains in chicken embryonic fibroblasts on the basis of the presence of some RNA species. To our knowledge, this study is the first to accomplish such a result, emphasizing how relevant a comprehensive picture of the viral transcriptome is to fully understand viral pathogenesis.",

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AU - Sadigh, Yashar

AU - Tahiri-Alaoui, Abdessamad

AU - Spatz, Stephen

AU - Nair, Venugopal

AU - Ribeca, Paolo

PY - 2020/3/18

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N2 - Marek’s disease is a major scourge challenging poultry health worldwide. It is caused by the highly contagious Marek’s disease virus (MDV), an alphaherpesvirus. Here, we showed that, similar to other members of its Herpesviridae family, MDV also presents a complex landscape of splicing events, most of which are uncharacterised and/or not annotated. Quite strikingly, and although the biological relevance of this fact is unknown, we found that a number of viral splicing isoforms are strain-specific, despite the close sequence similarity of the strains considered: very virulent RB-1B and vaccine CVI-988. We validated our findings by devising an assay that discriminated infections caused by the two strains in chicken embryonic fibroblasts on the basis of the presence of some RNA species. To our knowledge, this study is the first to accomplish such a result, emphasizing how relevant a comprehensive picture of the viral transcriptome is to fully understand viral pathogenesis.

AB - Marek’s disease is a major scourge challenging poultry health worldwide. It is caused by the highly contagious Marek’s disease virus (MDV), an alphaherpesvirus. Here, we showed that, similar to other members of its Herpesviridae family, MDV also presents a complex landscape of splicing events, most of which are uncharacterised and/or not annotated. Quite strikingly, and although the biological relevance of this fact is unknown, we found that a number of viral splicing isoforms are strain-specific, despite the close sequence similarity of the strains considered: very virulent RB-1B and vaccine CVI-988. We validated our findings by devising an assay that discriminated infections caused by the two strains in chicken embryonic fibroblasts on the basis of the presence of some RNA species. To our knowledge, this study is the first to accomplish such a result, emphasizing how relevant a comprehensive picture of the viral transcriptome is to fully understand viral pathogenesis.

KW - MDV strain CVI-988

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KW - Marek's disease virus

KW - RNA splicing isoforms

KW - Transcriptomics

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Pervasive Differential Splicing in Marek’s Disease Virus can Discriminate CVI-988 Vaccine Strain from RB-1B Very Virulent Strain in Chicken Embryonic Fibroblasts

Abstract

Bibliographical note

Funder

Funding

Keywords

ASJC Scopus subject areas

UN SDGs

Access to Document

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