PCR and molecular detection for differentiating Vibrio species

O. A.E. Sparagano; Peter A.W. Robertson; I. Purdom; J. McInnes; Y. Li; D. H. Yu; Z. J. Du; H. S. Xu; B. Austin

doi:10.1111/j.1749-6632.2002.tb04351.x

PCR and molecular detection for differentiating Vibrio species

O. A.E. Sparagano, Peter A.W. Robertson, I. Purdom, J. McInnes, Y. Li, D. H. Yu, Z. J. Du, H. S. Xu, B. Austin

Research output: Contribution to journal › Article › peer-review

10 Citations (Scopus)

Abstract

Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

Original language	English
Pages (from-to)	60-65
Number of pages	6
Journal	Annals of the New York Academy of Sciences
Volume	969
Issue number	1
DOIs	https://doi.org/10.1111/j.1749-6632.2002.tb04351.x
Publication status	Published - 1 Oct 2002
Externally published	Yes

Keywords

16S PCR reactions
23S PCR reactions
Aquaculture
Vibrio species
Vibriosis

ASJC Scopus subject areas

General Biochemistry,Genetics and Molecular Biology
History and Philosophy of Science

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1111/j.1749-6632.2002.tb04351.x

Cite this

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title = "PCR and molecular detection for differentiating Vibrio species",

abstract = "Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.",

keywords = "16S PCR reactions, 23S PCR reactions, Aquaculture, Vibrio species, Vibriosis",

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doi = "10.1111/j.1749-6632.2002.tb04351.x",

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T1 - PCR and molecular detection for differentiating Vibrio species

AU - Sparagano, O. A.E.

AU - Robertson, Peter A.W.

AU - Purdom, I.

AU - McInnes, J.

AU - Li, Y.

AU - Yu, D. H.

AU - Du, Z. J.

AU - Xu, H. S.

AU - Austin, B.

PY - 2002/10/1

Y1 - 2002/10/1

N2 - Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

AB - Vibriosis is an economically important disease of fish, marine invertebrates (particularly penaeid shrimps), and large marine mammals and is responsible for high mortality rates in aquaculture worldwide. Some Vibrio species are also responsible for zoonoses, whereas others are relatively nonpathogenic. Using 16S- and 23S-based PCR reactions, we obtained species-specific patterns and a 470-bp band, respectively. DNA sequences obtained on the 23S rRNA gene allowed us to identify species-specific probes for Vibrio parahaemolyticus, V. alginolyticus, V. anguillarum and for a cluster of taxonomically related species: V. carchariae/harveyi/campbelii. A phylogenetic tree based on the 23S sequences confirmed previous results obtained by Western blotting.

KW - 16S PCR reactions

KW - 23S PCR reactions

KW - Aquaculture

KW - Vibrio species

KW - Vibriosis

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DO - 10.1111/j.1749-6632.2002.tb04351.x

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AN - SCOPUS:0036411702

SN - 0077-8923

VL - 969

SP - 60

EP - 65

JO - Annals of the New York Academy of Sciences

JF - Annals of the New York Academy of Sciences

IS - 1

ER -

PCR and molecular detection for differentiating Vibrio species

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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