Oxidation of tertiary amine-derivatized surfaces to control protein adhesion

Dorota A. Dobrzanska, Amy L. Cooper, Christopher G. Dowson, Stephen D. Evans, David J. Fox, Benjamin R. Johnson, Caroline I. Biggs, Rajan K. Randev, Helena M. Stec, Paul C. Taylor, Andrew Marsh

Research output: Contribution to journalArticlepeer-review

13 Citations (Scopus)


Selective oxidation of ω-tertiary amine self-assembled thiol monolayers to tertiary amine N-oxides is shown to transform the adhesion of model proteins lysozyme and fibrinogen upon them. Efficient preparation of both secondary and tertiary linker amides as judged by X-ray photoelectron spectroscopy (XPS) and water droplet contact angle was achieved with an improved amide bond formation on gold quartz crystal microbalance (QCM) sensors using 2-(1H-7-azabenzotriazol-1-yl)-1,1,3,3-tetramethyl hexafluorophosphate methanaminium uronium (HATU). Oxidation with hydrogen peroxide was similarly assessed, and adhesion of lysozyme and fibrinogen from phosphate buffered saline was then assayed by QCM and imaged by AFM. Tertiary amine-functionalized sensors adsorbed multilayers of aggregated lysozyme, whereas tertiary amine N-oxides and triethylene glycol-terminated monolayers are consistent with small protein aggregates. The surface containing a dimethylamine N-oxide headgroup and ethyl secondary amide linker showed the largest difference in adsorption of both proteins. Oxidation of tertiary amine decorated surfaces therefore holds the potential for selective deposition of proteins and cells through masking and other patterning techniques.
Original languageEnglish
Pages (from-to)2961-2970
Number of pages10
Issue number9
Publication statusPublished - 12 Feb 2013


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