Osteoblast cell response to a CO2 laser modified polymeric material

D. G. Waugh, J. Lawrence, E. M. Brown

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Lasers are an efficient technology, which can be applied for the surface treatment of polymeric biomaterials to enhance insufficient surface properties. That is, the surface chemistry and topography of biomaterials can be modulated to increase the biofunctionality of that material. By employing CO2 laser patterning and whole area processing of nylon 6,6 this paper details how the surface properties were significantly modified. Samples, which had undergone whole area processing, followed the current theory in which the advancing contact angle, θ, with water decreased and the polar component, γp, increased upon an increase in surface roughness. For the patterned samples it was observed that θ increased and γP decreased. This did not follow the current theory and can be explained by a mixed-state wetting regime. By seeding osteoblast cells onto the samples for 24 h and 4 days the laser surface treatment gave rise to modulated cell response. For the laser whole area processing, θ and γP correlated with the observed cell count and cover density. Owed to the wetting regime, the patterned samples did not give rise to any correlative trend. As a result, CO2 laser whole area processing is more likely to allow one to predict biofunctionality prior to cell seeding. Moreover, for all samples, cell differentiation was evidenced. On account of this and the modulation in cell response, it has been shown that laser surface treatment lends itself to changing the biofunctional properties of nylon 6,6.

Original languageEnglish
Pages (from-to)236-247
Number of pages12
JournalOptics and Lasers in Engineering
Volume50
Issue number2
Early online date13 Oct 2011
DOIs
Publication statusPublished - 1 Feb 2012
Externally publishedYes

Fingerprint

osteoblasts
Osteoblasts
Lasers
Polymers
surface treatment
cells
lasers
Surface treatment
Nylon (trademark)
Biocompatible Materials
inoculation
Processing
Biomaterials
surface properties
wetting
Surface properties
Wetting
Surface topography
Surface chemistry
Contact angle

Bibliographical note

NOTICE: this is the author’s version of a work that was accepted for publication in Optics and Lasers in Engineering. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Optics and Lasers in Engineering, 50:2, (2012)
DOI: 10.1016/j.optlaseng.2011.08.010

© 2011, Elsevier. Licensed under the Creative Commons Attribution-NonCommercialNoDerivatives 4.0 International http://creativecommons.org/licenses/by-nc-nd/4.0/

Keywords

  • CO laser
  • Nylon 6,6
  • Osteoblast cells
  • Surface treatment
  • Wettability

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Mechanical Engineering
  • Electrical and Electronic Engineering

Cite this

Osteoblast cell response to a CO2 laser modified polymeric material. / Waugh, D. G.; Lawrence, J.; Brown, E. M.

In: Optics and Lasers in Engineering, Vol. 50, No. 2, 01.02.2012, p. 236-247.

Research output: Contribution to journalArticle

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