Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

Obolbek Turapov; Simon J Waddell; Bernard Burke; Sarah Glenn; Asel A Sarybaeva; Griselda Tudo; Gilles Labesse; Danielle I Young; Michael Young; Peter W Andrew; Philip D Butcher; Martin Cohen-Gonsaud; Galina V Mukamolova

doi:10.1074/jbc.M114.577338

Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

Obolbek Turapov, Simon J Waddell, Bernard Burke, Sarah Glenn, Asel A Sarybaeva, Griselda Tudo, Gilles Labesse, Danielle I Young, Michael Young, Peter W Andrew, Philip D Butcher, Martin Cohen-Gonsaud, Galina V Mukamolova

Research output: Contribution to journal › Article › peer-review

7 Citations (Scopus)

Abstract

We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

Original language	English
Pages (from-to)	25241-9
Number of pages	9
Journal	Journal of Biological Chemistry
Volume	289
Issue number	36
Early online date	10 Jul 2014
DOIs	https://doi.org/10.1074/jbc.M114.577338
Publication status	Published - 5 Sept 2014
Externally published	Yes

Keywords

Acyl Coenzyme A
Amino Acid Sequence
Bacterial Proteins
Binding Sites
DNA, Bacterial
Fluorescence Polarization
Gene Expression Regulation, Bacterial
Microbial Viability
Molecular Sequence Data
Molecular Structure
Mutation
Mycobacterium
Mycobacterium bovis
Mycobacterium tuberculosis
Oleic Acid
Palmitoyl Coenzyme A
Protein Binding
Reverse Transcriptase Polymerase Chain Reaction
Sequence Homology, Amino Acid
Transcriptome
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1074/jbc.M114.577338

Bernard Burke
- Centre for Health and Life Sciences - Associate Professor Research
Person: Teaching and Research

Cite this

Turapov, O., Waddell, S. J., Burke, B., Glenn, S., Sarybaeva, A. A., Tudo, G., Labesse, G., Young, D. I., Young, M., Andrew, P. W., Butcher, P. D., Cohen-Gonsaud, M., & Mukamolova, G. V. (2014). Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria. Journal of Biological Chemistry, 289(36), 25241-9. https://doi.org/10.1074/jbc.M114.577338

Turapov, O, Waddell, SJ, Burke, B, Glenn, S, Sarybaeva, AA, Tudo, G, Labesse, G, Young, DI, Young, M, Andrew, PW, Butcher, PD, Cohen-Gonsaud, M & Mukamolova, GV 2014, 'Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria', Journal of Biological Chemistry, vol. 289, no. 36, pp. 25241-9. https://doi.org/10.1074/jbc.M114.577338

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title = "Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria",

abstract = "We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Gu{\'e}rin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.",

keywords = "Acyl Coenzyme A, Amino Acid Sequence, Bacterial Proteins, Binding Sites, DNA, Bacterial, Fluorescence Polarization, Gene Expression Regulation, Bacterial, Microbial Viability, Molecular Sequence Data, Molecular Structure, Mutation, Mycobacterium, Mycobacterium bovis, Mycobacterium tuberculosis, Oleic Acid, Palmitoyl Coenzyme A, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Transcriptome, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",

author = "Obolbek Turapov and Waddell, {Simon J} and Bernard Burke and Sarah Glenn and Sarybaeva, {Asel A} and Griselda Tudo and Gilles Labesse and Young, {Danielle I} and Michael Young and Andrew, {Peter W} and Butcher, {Philip D} and Martin Cohen-Gonsaud and Mukamolova, {Galina V}",

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T1 - Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

AU - Turapov, Obolbek

AU - Waddell, Simon J

AU - Burke, Bernard

AU - Glenn, Sarah

AU - Sarybaeva, Asel A

AU - Tudo, Griselda

AU - Labesse, Gilles

AU - Young, Danielle I

AU - Young, Michael

AU - Andrew, Peter W

AU - Butcher, Philip D

AU - Cohen-Gonsaud, Martin

AU - Mukamolova, Galina V

PY - 2014/9/5

Y1 - 2014/9/5

N2 - We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

AB - We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

KW - Acyl Coenzyme A

KW - Amino Acid Sequence

KW - Bacterial Proteins

KW - Binding Sites

KW - DNA, Bacterial

KW - Fluorescence Polarization

KW - Gene Expression Regulation, Bacterial

KW - Microbial Viability

KW - Molecular Sequence Data

KW - Molecular Structure

KW - Mutation

KW - Mycobacterium

KW - Mycobacterium bovis

KW - Mycobacterium tuberculosis

KW - Oleic Acid

KW - Palmitoyl Coenzyme A

KW - Protein Binding

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Homology, Amino Acid

KW - Transcriptome

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1074/jbc.M114.577338

DO - 10.1074/jbc.M114.577338

M3 - Article

C2 - 25012658

SN - 0021-9258

SN - 1083-351X

VL - 289

SP - 25241

EP - 25249

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 36

ER -

Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

Abstract

Keywords

UN SDGs

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Bernard Burke

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