Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

Obolbek Turapov, Simon J Waddell, Bernard Burke, Sarah Glenn, Asel A Sarybaeva, Griselda Tudo, Gilles Labesse, Danielle I Young, Michael Young, Peter W Andrew, Philip D Butcher, Martin Cohen-Gonsaud, Galina V Mukamolova

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

Original languageEnglish
Pages (from-to)25241-9
Number of pages9
JournalJournal of Biological Chemistry
Volume289
Issue number36
Early online date10 Jul 2014
DOIs
Publication statusPublished - 5 Sep 2014
Externally publishedYes

Fingerprint

Acyl Coenzyme A
Mycobacterium
Pumps
Palmitoyl Coenzyme A
Derivatives
Regulon
DNA
Bacilli
Oleic Acid
Mycobacterium bovis
Mycobacterium tuberculosis
Bacillus
Genes
Adenosine Triphosphate
Gene Expression
Infection
oleoyl-coenzyme A

Keywords

  • Acyl Coenzyme A
  • Amino Acid Sequence
  • Bacterial Proteins
  • Binding Sites
  • DNA, Bacterial
  • Fluorescence Polarization
  • Gene Expression Regulation, Bacterial
  • Microbial Viability
  • Molecular Sequence Data
  • Molecular Structure
  • Mutation
  • Mycobacterium
  • Mycobacterium bovis
  • Mycobacterium tuberculosis
  • Oleic Acid
  • Palmitoyl Coenzyme A
  • Protein Binding
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sequence Homology, Amino Acid
  • Transcriptome
  • Journal Article
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

Cite this

Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria. / Turapov, Obolbek; Waddell, Simon J; Burke, Bernard; Glenn, Sarah; Sarybaeva, Asel A; Tudo, Griselda; Labesse, Gilles; Young, Danielle I; Young, Michael; Andrew, Peter W; Butcher, Philip D; Cohen-Gonsaud, Martin; Mukamolova, Galina V.

In: Journal of Biological Chemistry, Vol. 289, No. 36, 05.09.2014, p. 25241-9.

Research output: Contribution to journalArticle

Turapov, O, Waddell, SJ, Burke, B, Glenn, S, Sarybaeva, AA, Tudo, G, Labesse, G, Young, DI, Young, M, Andrew, PW, Butcher, PD, Cohen-Gonsaud, M & Mukamolova, GV 2014, 'Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria' Journal of Biological Chemistry, vol. 289, no. 36, pp. 25241-9. https://doi.org/10.1074/jbc.M114.577338
Turapov, Obolbek ; Waddell, Simon J ; Burke, Bernard ; Glenn, Sarah ; Sarybaeva, Asel A ; Tudo, Griselda ; Labesse, Gilles ; Young, Danielle I ; Young, Michael ; Andrew, Peter W ; Butcher, Philip D ; Cohen-Gonsaud, Martin ; Mukamolova, Galina V. / Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria. In: Journal of Biological Chemistry. 2014 ; Vol. 289, No. 36. pp. 25241-9.
@article{81fbaa2e092d4989ad760fe6d259e108,
title = "Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria",
abstract = "We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Gu{\'e}rin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.",
keywords = "Acyl Coenzyme A, Amino Acid Sequence, Bacterial Proteins, Binding Sites, DNA, Bacterial, Fluorescence Polarization, Gene Expression Regulation, Bacterial, Microbial Viability, Molecular Sequence Data, Molecular Structure, Mutation, Mycobacterium, Mycobacterium bovis, Mycobacterium tuberculosis, Oleic Acid, Palmitoyl Coenzyme A, Protein Binding, Reverse Transcriptase Polymerase Chain Reaction, Sequence Homology, Amino Acid, Transcriptome, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Obolbek Turapov and Waddell, {Simon J} and Bernard Burke and Sarah Glenn and Sarybaeva, {Asel A} and Griselda Tudo and Gilles Labesse and Young, {Danielle I} and Michael Young and Andrew, {Peter W} and Butcher, {Philip D} and Martin Cohen-Gonsaud and Mukamolova, {Galina V}",
note = "{\circledC} 2014 by The American Society for Biochemistry and Molecular Biology, Inc.",
year = "2014",
month = "9",
day = "5",
doi = "10.1074/jbc.M114.577338",
language = "English",
volume = "289",
pages = "25241--9",
journal = "Journal of Biological Chemistry",
issn = "0021-9258",
publisher = "American Society for Biochemistry and Molecular Biology",
number = "36",

}

TY - JOUR

T1 - Oleoyl coenzyme A regulates interaction of transcriptional regulator RaaS (Rv1219c) with DNA in mycobacteria

AU - Turapov, Obolbek

AU - Waddell, Simon J

AU - Burke, Bernard

AU - Glenn, Sarah

AU - Sarybaeva, Asel A

AU - Tudo, Griselda

AU - Labesse, Gilles

AU - Young, Danielle I

AU - Young, Michael

AU - Andrew, Peter W

AU - Butcher, Philip D

AU - Cohen-Gonsaud, Martin

AU - Mukamolova, Galina V

N1 - © 2014 by The American Society for Biochemistry and Molecular Biology, Inc.

PY - 2014/9/5

Y1 - 2014/9/5

N2 - We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

AB - We have recently shown that RaaS (regulator of antimicrobial-assisted survival), encoded by Rv1219c in Mycobacterium tuberculosis and by bcg_1279c in Mycobacterium bovis bacillus Calmette-Guérin, plays an important role in mycobacterial survival in prolonged stationary phase and during murine infection. Here, we demonstrate that long chain acyl-CoA derivatives (oleoyl-CoA and, to lesser extent, palmitoyl-CoA) modulate RaaS binding to DNA and expression of the downstream genes that encode ATP-dependent efflux pumps. Moreover, exogenously added oleic acid influences RaaS-mediated mycobacterial improvement of survival and expression of the RaaS regulon. Our data suggest that long chain acyl-CoA derivatives serve as biological indicators of the bacterial metabolic state. Dysregulation of efflux pumps can be used to eliminate non-growing mycobacteria.

KW - Acyl Coenzyme A

KW - Amino Acid Sequence

KW - Bacterial Proteins

KW - Binding Sites

KW - DNA, Bacterial

KW - Fluorescence Polarization

KW - Gene Expression Regulation, Bacterial

KW - Microbial Viability

KW - Molecular Sequence Data

KW - Molecular Structure

KW - Mutation

KW - Mycobacterium

KW - Mycobacterium bovis

KW - Mycobacterium tuberculosis

KW - Oleic Acid

KW - Palmitoyl Coenzyme A

KW - Protein Binding

KW - Reverse Transcriptase Polymerase Chain Reaction

KW - Sequence Homology, Amino Acid

KW - Transcriptome

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1074/jbc.M114.577338

DO - 10.1074/jbc.M114.577338

M3 - Article

VL - 289

SP - 25241

EP - 25249

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

SN - 0021-9258

IS - 36

ER -