Nanomechanical Contribution of Collagen and von Willebrand Factor A in Marine Underwater Adhesion and Its Implication for Collagen Manipulation

Hee Young Yoo, Jun Huang, Lin Li, Mathias Foo, Hongbo Zeng, Dong Soo Hwang

Research output: Contribution to journalArticle

7 Citations (Scopus)
11 Downloads (Pure)

Abstract

Recent works on mussel adhesion have identified a load bearing matrix protein (PTMP1) containing von Willebrand factor (vWF) with collagen binding capability that contributes to the mussel holdfast by manipulating mussel collagens. Using a surface forces apparatus, we investigate for the first time, the nanomechanical properties of vWF-collagen interaction using homologous proteins of mussel byssus, PTMP1 and preCollagens (preCols), as collagen. Mimicking conditions similar to mussel byssus secretion (pH <5.0) and seawater condition (pH 8.0), PTMP1 and preCol interact weakly in the "positioning" phase based on vWF-collagen binding and strengthen in "locked" phase due to the combined effects of electrostatic attraction, metal binding, and mechanical shearing. The progressive enhancement of binding between PTMP1 with porcine collagen under the aforementioned conditions is also observed. The binding mechanisms of PTMP1-preCols provide insights into the molecular interaction of the mammalian collagen system and the development of an artificial extracellular matrix based on collagens.
Original languageEnglish
Pages (from-to)946-953
Number of pages8
JournalBiomacromolecules
Volume17
Issue number3
Early online date19 Feb 2016
DOIs
Publication statusPublished - 14 Mar 2016

Bibliographical note

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Keywords

  • Proximal thread matrix protein-1;
  • Nanomechanics
  • von Willebrand factor (vWF);
  • Tissue engineering
  • Mussel adhesion
  • Shear effect

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