MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants

Steven Foster, Mikhajlo K. Zubko, Sandrine Guillard, David Lydall

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

MRX, an evolutionally conserved DNA damage response complex composed of Mre11, Rad50 and Xrs2, is involved in DNA double strand break (DSB) repair, checkpoint activation and telomere maintenance. At DSBs, MRX plays a role in generating single stranded DNA (ssDNA) and signalling cell cycle arrest. Here we investigated whether MRX also contributes to generating ssDNA or signalling cell cycle arrest at uncapped telomeres. To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein. We show that Rad50 does not contribute to ssDNA generation or cell cycle arrest in response to cdcl3-1 uncapped telomeres. Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping.
Original languageEnglish
Pages (from-to)840-851
Number of pages12
JournalDNA Repair
Volume5
Issue number7
Early online date12 Jun 2006
DOIs
Publication statusPublished - 13 Jul 2006

Fingerprint

Saccharomycetales
Single-Stranded DNA
Telomere
Yeast
Cell signaling
Cell Cycle Checkpoints
DNA
Cells
Double-Stranded DNA Breaks
Proteins
Repair
Chemical activation
DNA Damage
Cell Survival
Maintenance
Mutation
Temperature

Keywords

  • RAD50
  • MRX
  • Telomere
  • Checkpoint
  • ssDNE
  • CDC13

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants. / Foster, Steven; Zubko, Mikhajlo K.; Guillard, Sandrine; Lydall, David.

In: DNA Repair, Vol. 5, No. 7, 13.07.2006, p. 840-851.

Research output: Contribution to journalArticle

Foster, Steven ; Zubko, Mikhajlo K. ; Guillard, Sandrine ; Lydall, David. / MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants. In: DNA Repair. 2006 ; Vol. 5, No. 7. pp. 840-851.
@article{c848a2a6f1ae4f3f812e52fb7a47c0f0,
title = "MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants",
abstract = "MRX, an evolutionally conserved DNA damage response complex composed of Mre11, Rad50 and Xrs2, is involved in DNA double strand break (DSB) repair, checkpoint activation and telomere maintenance. At DSBs, MRX plays a role in generating single stranded DNA (ssDNA) and signalling cell cycle arrest. Here we investigated whether MRX also contributes to generating ssDNA or signalling cell cycle arrest at uncapped telomeres. To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein. We show that Rad50 does not contribute to ssDNA generation or cell cycle arrest in response to cdcl3-1 uncapped telomeres. Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping.",
keywords = "RAD50, MRX, Telomere, Checkpoint, ssDNE, CDC13",
author = "Steven Foster and Zubko, {Mikhajlo K.} and Sandrine Guillard and David Lydall",
year = "2006",
month = "7",
day = "13",
doi = "10.1016/j.dnarep.2006.04.005",
language = "English",
volume = "5",
pages = "840--851",
journal = "DNA Repair",
issn = "1568-7864",
publisher = "Elsevier",
number = "7",

}

TY - JOUR

T1 - MRX protects telomeric DNA at uncapped telomeres of budding yeast cdc13-1 mutants

AU - Foster, Steven

AU - Zubko, Mikhajlo K.

AU - Guillard, Sandrine

AU - Lydall, David

PY - 2006/7/13

Y1 - 2006/7/13

N2 - MRX, an evolutionally conserved DNA damage response complex composed of Mre11, Rad50 and Xrs2, is involved in DNA double strand break (DSB) repair, checkpoint activation and telomere maintenance. At DSBs, MRX plays a role in generating single stranded DNA (ssDNA) and signalling cell cycle arrest. Here we investigated whether MRX also contributes to generating ssDNA or signalling cell cycle arrest at uncapped telomeres. To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein. We show that Rad50 does not contribute to ssDNA generation or cell cycle arrest in response to cdcl3-1 uncapped telomeres. Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping.

AB - MRX, an evolutionally conserved DNA damage response complex composed of Mre11, Rad50 and Xrs2, is involved in DNA double strand break (DSB) repair, checkpoint activation and telomere maintenance. At DSBs, MRX plays a role in generating single stranded DNA (ssDNA) and signalling cell cycle arrest. Here we investigated whether MRX also contributes to generating ssDNA or signalling cell cycle arrest at uncapped telomeres. To investigate the role of MRX, we generated a conditionally degradable Rad50 protein and combined this with cdc13-1, a temperature sensitive mutation in the Cdc13 telomere capping protein. We show that Rad50 does not contribute to ssDNA generation or cell cycle arrest in response to cdcl3-1 uncapped telomeres. Instead, we find that Rad50 inhibits ssDNA accumulation and promotes cdc13-1 cell viability, consistent with a major role for MRX in telomere capping.

KW - RAD50

KW - MRX

KW - Telomere

KW - Checkpoint

KW - ssDNE

KW - CDC13

U2 - 10.1016/j.dnarep.2006.04.005

DO - 10.1016/j.dnarep.2006.04.005

M3 - Article

VL - 5

SP - 840

EP - 851

JO - DNA Repair

JF - DNA Repair

SN - 1568-7864

IS - 7

ER -