mRNA levels are buffered upon knockdown of RNA decay and translation factors via adjustment of transcription rates in human HepG2 cells

Pavneet Singh, Rob James, Christopher Mee, Igor Morozov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Evidence from yeast and mammals argues the existence of cross-talk between transcription and mRNA decay. Stabilization of transcripts upon depletion of mRNA decay factors generally leads to no changes in mRNA abundance, attributing this to decreased transcription rates. We show that knockdown of human XRN1, CNOT6 and ETF1 genes in HepG2 cells led to significant alteration in stability of specific mRNAs, alterations in half-life were inversely-associated with transcription rates, mostly not resulting in changes in abundance. We demonstrate the existence of the gene expression buffering mechanism in human cells that responds to both transcript stabilization and destabilization to maintain mRNA abundance via altered transcription rates and may involve translation. We propose that this buffering may hold novel cancer therapeutic targets.
Original languageEnglish
Pages (from-to)1147-1155
Number of pages9
JournalRNA Biology
Volume16
Issue number9
Early online date31 May 2019
DOIs
Publication statusPublished - 2 Sep 2019

Keywords

  • Gene expression, Human cells, cancer, coupling, mRNA decay, transcription, translation, regulation
  • Human cells
  • Cancer
  • Coupling
  • mRNA decay
  • Transcription
  • Translation
  • Regulation

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