Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP

Alberto Alberti, Olivier A.E. Sparagano

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

16 Citations (Scopus)

Abstract

Anaplasma phagocytophilum (A. phagocytophilum, formerly Ehrlichia phagocytophila) is a tick-borne pathogen responsible for tick-borne fever in ruminants, equine granulocytic ehrlichiosis (EGE) in horses, canine granulocytic ehrlichiosis (CGE) in dogs, and for human granulocytic ehrlichiosis (HGE). Human cases have been registered in many countries with a broad range of symptoms and pathogenicity. This article focused on Sardinia as the prevalence in humans was almost seven times higher than in the rest of Italy. To evaluate the risk, blood samples were collected from dogs and horses on the island. Genomic DNA was extracted from the buffy coat and amplified by heminested polymerase chain reaction (PCR) using the groEL gene primers. The first PCR reaction amplified a 624-bp fragment for both A. phagocytophilum and A. platys while the second PCR reaction amplified 573-bp and 515-bp fragments for the above two pathogens, respectively. Six A. phagocytophilum samples were PCR positive (3 dogs and 3 horses) while another dog was A. platys PCR positive. A phylogenetic analysis was conducted with A. phagocytophilum sequences in GenBank from the United States, Slovenia, Switzerland, Germany, UK, Austria, and Czech Republic. Surprisingly, the related phylogenetic tree showed that the Sardinian isolates were closer to the American isolates, which were showing highest mortality rates than from the other two European lineages.

Original languageEnglish
Title of host publicationImpact of Emerging Zoonotic Diseases on Animal Health
Subtitle of host publication8th Biennial Conference of the Society for Tropical Veterinary Medicine
PublisherWiley
Pages371-378
Number of pages8
Volume1081
ISBN (Print)1573316377, 9781573316378
DOIs
Publication statusPublished - 1 Jan 2006
Externally publishedYes

Publication series

NameAnnals of the New York Academy of Sciences
Volume1081
ISSN (Print)0077-8923
ISSN (Electronic)1749-6632

Fingerprint

Anaplasmosis
Anaplasma phagocytophilum
Polymerase chain reaction
Restriction Fragment Length Polymorphisms
Ehrlichiosis
Polymerase Chain Reaction
Horses
Dogs
Pathogens
Ticks
Italy
Slovenia
Austria
Czech Republic
Nucleic Acid Databases
Ruminants
Switzerland
Islands
Blood
Genes

Keywords

  • Anaplasmosis
  • Dog
  • Ehrlichiosis
  • Horse
  • Italy
  • PCR

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Alberti, A., & Sparagano, O. A. E. (2006). Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP. In Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine (Vol. 1081, pp. 371-378). (Annals of the New York Academy of Sciences; Vol. 1081). Wiley. https://doi.org/10.1196/annals.1373.055

Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP. / Alberti, Alberto; Sparagano, Olivier A.E.

Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. Vol. 1081 Wiley, 2006. p. 371-378 (Annals of the New York Academy of Sciences; Vol. 1081).

Research output: Chapter in Book/Report/Conference proceedingConference proceeding

Alberti, A & Sparagano, OAE 2006, Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP. in Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. vol. 1081, Annals of the New York Academy of Sciences, vol. 1081, Wiley, pp. 371-378. https://doi.org/10.1196/annals.1373.055
Alberti A, Sparagano OAE. Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP. In Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. Vol. 1081. Wiley. 2006. p. 371-378. (Annals of the New York Academy of Sciences). https://doi.org/10.1196/annals.1373.055
Alberti, Alberto ; Sparagano, Olivier A.E. / Molecular diagnosis of granulocytic anaplasmosis and infectious cyclic thrombocytopenia by PCR-RFLP. Impact of Emerging Zoonotic Diseases on Animal Health: 8th Biennial Conference of the Society for Tropical Veterinary Medicine. Vol. 1081 Wiley, 2006. pp. 371-378 (Annals of the New York Academy of Sciences).
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