TY - JOUR
T1 - Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization
AU - Sparagano, Olivier A.E.
AU - De Vos, Alexander P.
AU - Paoletti, Barbara
AU - Cammà, Cesare
AU - De Santis, Paola
AU - Otranto, Domenico
AU - Giangaspero, Annunziata
PY - 2003/1/1
Y1 - 2003/1/1
N2 - Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.
AB - Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.
UR - http://www.scopus.com/inward/record.url?scp=0348216477&partnerID=8YFLogxK
U2 - 10.1177/104063870301500604
DO - 10.1177/104063870301500604
M3 - Article
C2 - 14667015
AN - SCOPUS:0348216477
SN - 1040-6387
VL - 15
SP - 527
EP - 534
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
IS - 6
ER -