Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization

Olivier A.E. Sparagano, Alexander P. De Vos, Barbara Paoletti, Cesare Cammà, Paola De Santis, Domenico Otranto, Annunziata Giangaspero

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.

Original languageEnglish
Pages (from-to)527-534
Number of pages8
JournalJournal of Veterinary Diagnostic Investigation
Volume15
Issue number6
DOIs
Publication statusPublished - 1 Jan 2003
Externally publishedYes

Fingerprint

Anaplasma
Anaplasma platys
hybridization
polymerase chain reaction
Dogs
Polymerase Chain Reaction
dogs
Ehrlichia canis
Rhipicephalus sanguineus
Nucleic Acid Databases
Ticks
dog breeds
Italy
ticks
methodology
Infection
infection
sampling

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Sparagano, O. A. E., De Vos, A. P., Paoletti, B., Cammà, C., De Santis, P., Otranto, D., & Giangaspero, A. (2003). Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. Journal of Veterinary Diagnostic Investigation, 15(6), 527-534. https://doi.org/10.1177/104063870301500604

Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. / Sparagano, Olivier A.E.; De Vos, Alexander P.; Paoletti, Barbara; Cammà, Cesare; De Santis, Paola; Otranto, Domenico; Giangaspero, Annunziata.

In: Journal of Veterinary Diagnostic Investigation, Vol. 15, No. 6, 01.01.2003, p. 527-534.

Research output: Contribution to journalArticle

Sparagano, Olivier A.E. ; De Vos, Alexander P. ; Paoletti, Barbara ; Cammà, Cesare ; De Santis, Paola ; Otranto, Domenico ; Giangaspero, Annunziata. / Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization. In: Journal of Veterinary Diagnostic Investigation. 2003 ; Vol. 15, No. 6. pp. 527-534.
@article{b9aa175138c14a6890cba80fa03ae6c9,
title = "Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization",
abstract = "Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.",
author = "Sparagano, {Olivier A.E.} and {De Vos}, {Alexander P.} and Barbara Paoletti and Cesare Camm{\`a} and {De Santis}, Paola and Domenico Otranto and Annunziata Giangaspero",
year = "2003",
month = "1",
day = "1",
doi = "10.1177/104063870301500604",
language = "English",
volume = "15",
pages = "527--534",
journal = "Journal of Veterinary Diagnostic Investigation",
issn = "1040-6387",
publisher = "SAGE Publications",
number = "6",

}

TY - JOUR

T1 - Molecular detection of Anaplasma platys in dogs using polymerase chain reaction and reverse line blot hybridization

AU - Sparagano, Olivier A.E.

AU - De Vos, Alexander P.

AU - Paoletti, Barbara

AU - Cammà, Cesare

AU - De Santis, Paola

AU - Otranto, Domenico

AU - Giangaspero, Annunziata

PY - 2003/1/1

Y1 - 2003/1/1

N2 - Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.

AB - Several polymerase chain reactions (PCRs) and a reverse line blot hybridization (RLB) method were used to identify Anaplasma platys in dogs held in a kennel in Italy. Whereas PCR techniques confirmed the presence of A. platys, the RLB method not only correlated the results obtained by PCR but also ruled out the presence of other species such as Ehrlichia canis or E. chaffeensis. There was no correlation between infection status and age or breed of the dogs. Polymerase chain reaction performed on the Rhipicephalus sanguineus ticks collected from those dogs showed that they were also infected with A. platys. Sequences obtained from some samples and compared with those within the GenBank also confirmed the presence of A. platys.

UR - http://www.scopus.com/inward/record.url?scp=0348216477&partnerID=8YFLogxK

U2 - 10.1177/104063870301500604

DO - 10.1177/104063870301500604

M3 - Article

VL - 15

SP - 527

EP - 534

JO - Journal of Veterinary Diagnostic Investigation

JF - Journal of Veterinary Diagnostic Investigation

SN - 1040-6387

IS - 6

ER -