Method of preparing polypeptides in cell-free translation system

July B. Alakhov; Vladimir I. Baranov; Serei J. Ovodov; Ljubov A. Ryabova; Alexandr S. Spirin; Igor Morozov

Method of preparing polypeptides in cell-free translation system

July B. Alakhov (Inventor), Vladimir I. Baranov (Inventor), Serei J. Ovodov (Inventor), Ljubov A. Ryabova (Inventor), Alexandr S. Spirin (Inventor), Igor Morozov (Inventor)

Research output: Patent

Abstract

Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.

Original language	English
Patent number	US5478730A
IPC	C12P 21/00,C12N 9/00,C12Q 1/68
Priority date	21/12/88
Filing date	16/12/92
Publication status	Published - 26 Dec 1995

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title = "Method of preparing polypeptides in cell-free translation system",

abstract = "Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.",

author = "Alakhov, {July B.} and Baranov, {Vladimir I.} and Ovodov, {Serei J.} and Ryabova, {Ljubov A.} and Spirin, {Alexandr S.} and Igor Morozov",

year = "1995",

month = dec,

day = "26",

language = "English",

type = "Patent",

note = "US5478730A; C12P 21/00,C12N 9/00,C12Q 1/68",

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TY - PAT

T1 - Method of preparing polypeptides in cell-free translation system

AU - Alakhov, July B.

AU - Baranov, Vladimir I.

AU - Ovodov, Serei J.

AU - Ryabova, Ljubov A.

AU - Spirin, Alexandr S.

AU - Morozov, Igor

PY - 1995/12/26

Y1 - 1995/12/26

N2 - Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.

AB - Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.

M3 - Patent

M1 - US5478730A

Y2 - 1992/12/16

ER -

Method of preparing polypeptides in cell-free translation system

Abstract

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