Method of preparing polypeptides in cell-free translation system

July B. Alakhov (Inventor), Vladimir I. Baranov (Inventor), Serei J. Ovodov (Inventor), Ljubov A. Ryabova (Inventor), Alexandr S. Spirin (Inventor), Igor Morozov (Inventor)

Research output: Patent

Abstract

Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.
Original languageEnglish
Patent numberUS5478730
Priority date26/12/95
Filing date16/12/92
Publication statusPublished - 1992

Fingerprint

translation (genetics)
polypeptides
molecular weight
cell free system
cells
methodology
peptides
RNA
synthesis
cell-free protein synthesis

Cite this

Alakhov, J. B., Baranov, V. I., Ovodov, S. J., Ryabova, L. A., Spirin, A. S., & Morozov, I. (1992). Method of preparing polypeptides in cell-free translation system. (Patent No. US5478730).

Method of preparing polypeptides in cell-free translation system. / Alakhov, July B. (Inventor); Baranov, Vladimir I. (Inventor); Ovodov, Serei J. (Inventor); Ryabova, Ljubov A. (Inventor); Spirin, Alexandr S. (Inventor); Morozov, Igor (Inventor).

Patent No.: US5478730. Dec 16, 1992.

Research output: Patent

Alakhov, JB, Baranov, VI, Ovodov, SJ, Ryabova, LA, Spirin, AS & Morozov, I Dec. 16 1992, Method of preparing polypeptides in cell-free translation system, Patent No. US5478730.
Alakhov JB, Baranov VI, Ovodov SJ, Ryabova LA, Spirin AS, Morozov I, inventors. Method of preparing polypeptides in cell-free translation system. US5478730. 1992.
Alakhov, July B. (Inventor) ; Baranov, Vladimir I. (Inventor) ; Ovodov, Serei J. (Inventor) ; Ryabova, Ljubov A. (Inventor) ; Spirin, Alexandr S. (Inventor) ; Morozov, Igor (Inventor). / Method of preparing polypeptides in cell-free translation system. Patent No.: US5478730. Dec 16, 1992.
@misc{33ba2a1a8cc148449577502e4f9a3731,
title = "Method of preparing polypeptides in cell-free translation system",
abstract = "Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.",
author = "Alakhov, {July B.} and Baranov, {Vladimir I.} and Ovodov, {Serei J.} and Ryabova, {Ljubov A.} and Spirin, {Alexandr S.} and Igor Morozov",
year = "1992",
language = "English",
type = "Patent",
note = "US5478730",

}

TY - PAT

T1 - Method of preparing polypeptides in cell-free translation system

AU - Alakhov, July B.

AU - Baranov, Vladimir I.

AU - Ovodov, Serei J.

AU - Ryabova, Ljubov A.

AU - Spirin, Alexandr S.

AU - Morozov, Igor

PY - 1992

Y1 - 1992

N2 - Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.

AB - Cell-free production of protein is provided by employing a barrier which defines a reaction region for maintaining the high molecular weight components of the cell-free system while providing means for replenishing loss of low molecular weight components and removing low molecular weight products. Various configurations may be employed for continuous removal and addition of material during the polypeptide synthesis. Conveniently, porous membranes, hollow fibers, or other barriers may be employed. High ratios of peptide to RNA template result.

UR - https://patentimages.storage.googleapis.com/48/16/ca/893ab615d893ea/US5478730.pdf

M3 - Patent

M1 - US5478730

Y2 - 1992/12/16

ER -