Method of preparative genes expression in the cell-free system of coupled transcription/translation

Igor Morozov (Inventor)

    Research output: Patent

    Abstract

    Prodn. of polypeptides is effected by expression of genes in the form of DNA mols. in a cell-free transcription/translation system contg. amino acids, ATP, GTP, CTP and UTP as substrates, resulting in the formation of prods. including polypeptides, AMP, ADP, GDP, CDP, UDP, pyrophosphates and inorganic phosphates. The process is operated continuously, where the prods. are withdrawn as they are formed and the substrates are added so as to maintain their initial concns.
    Original languageEnglish
    Patent numberSU1705302A1
    Priority date22/12/88
    Filing date22/12/88
    Publication statusPublished - 15 Jan 1992

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